江西农业大学学报
江西農業大學學報
강서농업대학학보
ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS
2013年
6期
1248-1254
,共7页
肖莉春%孔祥峰%黄名钱%郭小权
肖莉春%孔祥峰%黃名錢%郭小權
초리춘%공상봉%황명전%곽소권
耐药性%大肠杆菌%中药%抑制作用
耐藥性%大腸桿菌%中藥%抑製作用
내약성%대장간균%중약%억제작용
antibiotic-resistance%E.Coli%Chinese herbs%inhibitory effect
筛选对耐药大肠杆菌具有抑制作用的中药，为抑菌中药方剂的研制提供依据。分别使用水提法和中性醇提法制备29味中药的提取物，并用打孔法、倍比稀释法测定其对10个猪源耐药大肠杆菌分离株和3个标准菌株的抑制活性。结果表明：五倍子、连翘和山楂的提取物对13株受试菌均有抑制作用，地榆、黄芩、石榴皮、秦皮、黄连、大黄、栀子和大青叶的提取物对部分菌株均有抑制活性；五倍子醇提物的抑菌圈直径大于20 mm，连翘提取物的抑菌圈直径均介于10～14 mm；五倍子醇提物对13个菌株的抑菌效果最佳，MIC值为15．6 mg/mL，而其水提物的MIC值为125～250 mg/mL；连翘提取物的抑菌效果次之，MIC值介于62．5～125 mg/mL；山楂醇提物对13个菌株的MIC值介于125～250 mg/mL，而其水提物除对SEC616、SEC911、C193、C197外的其它菌株的MIC值均介于250～500 mg/mL；地榆、黄芩、秦皮和大黄的提取物的MIC值均偏高，大青叶水提物的MIC值为250或500 mg/mL。实验得出：五倍子和连翘对13株受试菌的抑制作用最强，山楂、秦皮、大黄、大青叶、黄连和黄芩对部分受试菌也有一定抑制作用。
篩選對耐藥大腸桿菌具有抑製作用的中藥，為抑菌中藥方劑的研製提供依據。分彆使用水提法和中性醇提法製備29味中藥的提取物，併用打孔法、倍比稀釋法測定其對10箇豬源耐藥大腸桿菌分離株和3箇標準菌株的抑製活性。結果錶明：五倍子、連翹和山楂的提取物對13株受試菌均有抑製作用，地榆、黃芩、石榴皮、秦皮、黃連、大黃、梔子和大青葉的提取物對部分菌株均有抑製活性；五倍子醇提物的抑菌圈直徑大于20 mm，連翹提取物的抑菌圈直徑均介于10～14 mm；五倍子醇提物對13箇菌株的抑菌效果最佳，MIC值為15．6 mg/mL，而其水提物的MIC值為125～250 mg/mL；連翹提取物的抑菌效果次之，MIC值介于62．5～125 mg/mL；山楂醇提物對13箇菌株的MIC值介于125～250 mg/mL，而其水提物除對SEC616、SEC911、C193、C197外的其它菌株的MIC值均介于250～500 mg/mL；地榆、黃芩、秦皮和大黃的提取物的MIC值均偏高，大青葉水提物的MIC值為250或500 mg/mL。實驗得齣：五倍子和連翹對13株受試菌的抑製作用最彊，山楂、秦皮、大黃、大青葉、黃連和黃芩對部分受試菌也有一定抑製作用。
사선대내약대장간균구유억제작용적중약，위억균중약방제적연제제공의거。분별사용수제법화중성순제법제비29미중약적제취물，병용타공법、배비희석법측정기대10개저원내약대장간균분리주화3개표준균주적억제활성。결과표명：오배자、련교화산사적제취물대13주수시균균유억제작용，지유、황금、석류피、진피、황련、대황、치자화대청협적제취물대부분균주균유억제활성；오배자순제물적억균권직경대우20 mm，련교제취물적억균권직경균개우10～14 mm；오배자순제물대13개균주적억균효과최가，MIC치위15．6 mg/mL，이기수제물적MIC치위125～250 mg/mL；련교제취물적억균효과차지，MIC치개우62．5～125 mg/mL；산사순제물대13개균주적MIC치개우125～250 mg/mL，이기수제물제대SEC616、SEC911、C193、C197외적기타균주적MIC치균개우250～500 mg/mL；지유、황금、진피화대황적제취물적MIC치균편고，대청협수제물적MIC치위250혹500 mg/mL。실험득출：오배자화련교대13주수시균적억제작용최강，산사、진피、대황、대청협、황련화황금대부분수시균야유일정억제작용。
This study was conducted to screen out some Chinese herbs with strong anti MICrobial activity and provide basis for developing bacteriostatic Chinese berbal compounds .The aqueous and neutral ethanol ex-tracts of 29 kinds of Chinese herbs were prepared ,respectively,and then their antiMICrobial activities against 10 isolated strains of antibiotic-resistant E.Coli and 3 type strains of E.Coli were evaluated by determining di-ameter of inhibition zone ( IZD,mm) and minimal inhibitory concentration ( MIC,mg/mL) .Three extracts from Galla Chinensis,Fructus forsythiae and Fructus crataegi had antiMICrobialactivity to 13 strains of the tested E. Coli, and extracts from Sanguisorba officinalis,Scutellaria baicalensis,Punica granatum,Fraxinus rhynchophylla,Coptis chinensis,Rheum officinale,Gardenia jasminoides,and Folium isatidis had antimicrobial activity to some of the tested strains;the IZD of ethanol extract from Galla chinensis was higher than 20 mm against all of the tested strains,and that of two extracts from Fructus forsythiae were 10.0 to 14.0 mm.The MIC value of the etha-nol extract from Galla chinensis was 15.6 mg/mL,while that of its aqueous extract was 125 to 250 mg/mL a-gainst 13 tested strains,which indicated that the ethanol extract had the strongest anti MICrobial activity;the two extracts of Forsythia suspensa with MIC values of 125 to 250 mg/mL had a secondary anti MICrobial activi-ty;the MIC values of the ethanol extract from Fructus crataegi was 125 to 250 mg/mL against 13 tested strains , and that of its aqueous extract was 250 to 500 mm against the tested strains except for SEC 616, SEC911, C193,and C197.Sanguisorba officinalis,Scutellaria baicalensis,Fraxinus rhynchophylla,and Rheum officinale had higher MIC values.The MIC value of the aqueous extract from Folium isatidis was 250 to 500 mg/mL.Ex-tracts from Galla chinensis and Forsythia suspensa had strongest anti MICrobial activity against 13 tested E.Coli strains,and the extracts from Fructus crataegi,Fraxinus rhynchophylla,Rheum officinale,Folium isatidis,Coptis chinensis,and Scutellaria baicalensis had activity to some of the tested strains .
