CAJ | 학술논문

为挖掘核桃内生菌的生物防治潜能。对核桃的内生菌和病原菌进行了分离，并通过平板对峙和发酵液抑菌试验，对其体外互作进行了研究。结果发现3株核桃内生细菌XHE7、XHE8和XHE12对2种病原真菌HK2（ Colletotrichium sp．）和HD1（ Alternaria sp．）有拮抗作用，抑制率高达82％。其发酵液对HK2的抑制作用高达100％。对其中2株拮抗作用明显的细菌进行了生理生化和16S rDNA分子鉴定，初步确定XHE8和XHE12皆为芽孢杆菌（ Bacillus sp．）。
위알굴핵도내생균적생물방치잠능。대핵도적내생균화병원균진행료분리，병통과평판대치화발효액억균시험，대기체외호작진행료연구。결과발현3주핵도내생세균XHE7、XHE8화XHE12대2충병원진균HK2（ Colletotrichium sp．）화HD1（ Alternaria sp．）유길항작용，억제솔고체82％。기발효액대HK2적억제작용고체100％。대기중2주길항작용명현적세균진행료생리생화화16S rDNA분자감정，초보학정XHE8화XHE12개위아포간균（ Bacillus sp．）。
In order to exploring the biocontrol talent of walnut endophytic bacteria ,several endophytes and two pathogens in walnut were isolated , and the in vitro interactions between endophyte and pathogens were studied by using plate-confrontation and zymotic fluid inhibition assay .The results showed that three endophyt-ic strains XHE7,XHE8 and XHE12 represented significant antagonism to both HK2(Colletotrichium sp.) and HD1 (Alternaria sp.),and the highest inhibition rate reached 82.01%.Their zymotic fluid could suppress HK2 growth rate as high as 100%.Combined physiological and biochemical characters with 16S rDNA sequencing results,both strains XHE8 and XHE12 were identified as Bacillus sp..