CAJ | 학술논문

目的：研究三氧化二砷（ As2 O3）对人腺样囊性癌ACC-2裸鼠移植瘤生长及血管生成的抑制作用，探讨其抗肿瘤的作用机制。方法通过建立ACC-2裸鼠移植瘤模型，将28只荷瘤鼠随机分为4组：阴性对照组（生理盐水即 NS ）、阳性对照组[5-Fu 10 mg/（ kg · d ）]、低剂量组[ As2 O32．5 mg/（kg· d）]、高剂量组[As2O35．0 mg/（kg· d）]。连续经腹腔注射用药10天。测量肿瘤的体积和重量，采用HE染色光镜观察，免疫组织化学法检测VEGF、MVD的表达并进行统计学分析。结果高、低剂量As2 O3组移植瘤的瘤体积、瘤重均低于两对照组（ NS组和5-Fu组），且高剂量As2 O3组抑瘤最明显，瘤体积抑制率为52．17％，瘤重抑制率为56．04％（ P＜0．01）。在移植瘤表面，与两对照组相比，高、低剂量As2 O3组血管密度减小，管径变细。高、低剂量As2 O3组抑制移植瘤VEGF的表达、降低MVD值，高剂量As2 O3组抑制作用最明显。与两对照组比较以及高、低剂量As2 O3组之间比较差异均有统计学意义（P＜0．01）。结论 As2O3对人腺样囊性癌ACC-2裸鼠移植瘤及血管生成有明显抑制作用，其作用机制可能为降低VEGF表达及MVD值，此抑制作用与剂量有关。
목적：연구삼양화이신（ As2 O3）대인선양낭성암ACC-2라서이식류생장급혈관생성적억제작용，탐토기항종류적작용궤제。방법통과건립ACC-2라서이식류모형，장28지하류서수궤분위4조：음성대조조（생리염수즉 NS ）、양성대조조[5-Fu 10 mg/（ kg · d ）]、저제량조[ As2 O32．5 mg/（kg· d）]、고제량조[As2O35．0 mg/（kg· d）]。련속경복강주사용약10천。측량종류적체적화중량，채용HE염색광경관찰，면역조직화학법검측VEGF、MVD적표체병진행통계학분석。결과고、저제량As2 O3조이식류적류체적、류중균저우량대조조（ NS조화5-Fu조），차고제량As2 O3조억류최명현，류체적억제솔위52．17％，류중억제솔위56．04％（ P＜0．01）。재이식류표면，여량대조조상비，고、저제량As2 O3조혈관밀도감소，관경변세。고、저제량As2 O3조억제이식류VEGF적표체、강저MVD치，고제량As2 O3조억제작용최명현。여량대조조비교이급고、저제량As2 O3조지간비교차이균유통계학의의（P＜0．01）。결론 As2O3대인선양낭성암ACC-2라서이식류급혈관생성유명현억제작용，기작용궤제가능위강저VEGF표체급MVD치，차억제작용여제량유관。
Objective This study is to investigate the inhibitory effect of Arsenic Trioxide ( As2O3 ) on salivary adenoid cystic carcinoma -2(ACC-2)cell xenografts and angiogenesis in nude mice .Methods The nude mice with ACC-2 cell xenografts were randomly distributed into four groups ,control normal saline ( NS) ,5-Fu group[5-Fu10 mg/(kg· d)],low-dose group[As2O32.5 mg/(kg· d)],and high-dose group[As2O3 5.0 mg/(kg· d)].0.2 mL NS or drugs were given by intraperitoneal injection for 10 days.Tumor volume and weight were measured ,the microstructure was observed by HE staining ,the expression of VEGF and MVD were detected by immunohistochemistry .Results The average tumor volume was significantly smaller and the average tumor weight was significantly lighter in As 2 O3 groups than in control groups ,especially in the high-dose group, the inhibitory rate were 52.17%and 56.04%(P<0.01).Compared with the control group,the MVD of treat-ment group was markedly decreased ,the pipe diameters were smaller in treatment group .The expression levels of VEGF and MVD were significantly lower in As 2 O3 groups than in control group , especially in the high -dose group.Conclusion As2O3 has obviously inhibitory effects on growth of ACC -2 cell xenografts and angiogenesis in nude mice by suppressing the expression of VEGF and MVD .The anticancer effect of As 2 O3 is related to the As2O3 concentration .