CAJ | 학술논문

目的：研究人参皂苷Rg3延缓乳腺癌三苯氧胺（ TAM）耐药的作用，并探究其最佳作用浓度。方法 TAM诱导野生型MCF-7乳腺癌细胞构建TAM耐药细胞系TAM-R，将WT-MCF-7乳腺癌细胞与TAM-R细胞分别培养于不同浓度TAM培养液中，MTT法比较细胞生长变化。 WT-MCF-7分别培养于含不同浓度Rg3的TAM培养液中，MTT法检测细胞对TAM的反应。结果经TAM处理后， TAM-R细胞生长曲线明显高于WT-MCF-7细胞的生长曲线，人参皂甙Rg3可延缓WT-MCF-7细胞TAM耐药的发生时间，1×10-8 mol/L和5×10-8 mol/L人参皂甙Rg3延缓耐药时间最长。结论临床可通过联合应用参一胶囊和TAM延缓内分泌耐药。
목적：연구인삼조감Rg3연완유선암삼분양알（ TAM）내약적작용，병탐구기최가작용농도。방법 TAM유도야생형MCF-7유선암세포구건TAM내약세포계TAM-R，장WT-MCF-7유선암세포여TAM-R세포분별배양우불동농도TAM배양액중，MTT법비교세포생장변화。 WT-MCF-7분별배양우함불동농도Rg3적TAM배양액중，MTT법검측세포대TAM적반응。결과경TAM처리후， TAM-R세포생장곡선명현고우WT-MCF-7세포적생장곡선，인삼조대Rg3가연완WT-MCF-7세포TAM내약적발생시간，1×10-8 mol/L화5×10-8 mol/L인삼조대Rg3연완내약시간최장。결론림상가통과연합응용삼일효낭화TAM연완내분비내약。
Objective To evaluate the effect and the best concentration of Rg 3 combined with delaying tamoxifen resistance in breast cancer .Methods The tamoxifen-resistant(TAM-R)cell line with TAM was es-tablished.TAM-R cell line and WT -MCF-7 cell line were cultured in TAM at different concentrations and then detected the growth of cells .WT-MCF-7 cell line was cultured medium in TAM which contained Rg 3 at different levels .The growing situation of cells was detected with MTT method .Results Compared with wild -type WT-MCF-7 cells,TAM-R showed higher curves when they were both at TAM treatment .Rg3 could be associated with delaying the emergency of tamoxifen resistance and the best concentration was 1 ×10 -8 mol/L and 5 ×10 -8 mol/L.Conclusion It might be useful to reduce the tamoxifen resistance incidence by clinically com-bined Rg3 with TAM treatment .