CAJ | 학술논문

为了明确转基因抗草甘膦棉花10-6001对除草剂草甘膦的耐受性，以转基因抗草甘膦棉花材料10-6001和常规棉花品种冀棉958（ CK）为试验材料，在棉苗6片真叶时叶面喷施不同浓度〔0（不喷施除草剂，人工除草， CK）、0.25％和0.50％〕的草甘膦溶液，分别于施药后0（当天下午）、1、7、14和21 d调查棉花的株高、药害级别、受害指数和死苗率，施药后35 d 调查棉花的死苗情况；分析2种棉花对不同浓度草甘膦的反应程度，并对其株高差异进行了显著性方差分析。结果表明：转基因抗草甘膦棉花品系10-6001对草甘膦的耐受性很好，喷施0.25％和0.50％草甘膦溶液的棉花在整个试验期间药害均较轻，表现为植株矮化，生长减慢，叶片略变小，且无死苗，株高方差分析结果显示与人工除草（ CK）相比差异均不显著；随着时间的延长，药害逐渐缓解。而常规品种冀棉958对草甘膦的耐受性很差，喷施0.25％和0.50％草甘膦溶液的棉花药害症状表现为植株矮化，生长缓慢，叶片变小、枯黄、褐化干枯，并且药害程度随着时间的延长而加重，药害级别和受害指数均高于10-6001，株高方差分析结果显示与人工除草（ CK）相比差异均达到了极显著水平，施药后35 d部分棉花出现整株枯死。
위료명학전기인항초감련면화10-6001대제초제초감련적내수성，이전기인항초감련면화재료10-6001화상규면화품충기면958（ CK）위시험재료，재면묘6편진협시협면분시불동농도〔0（불분시제초제，인공제초， CK）、0.25％화0.50％〕적초감련용액，분별우시약후0（당천하오）、1、7、14화21 d조사면화적주고、약해급별、수해지수화사묘솔，시약후35 d 조사면화적사묘정황；분석2충면화대불동농도초감련적반응정도，병대기주고차이진행료현저성방차분석。결과표명：전기인항초감련면화품계10-6001대초감련적내수성흔호，분시0.25％화0.50％초감련용액적면화재정개시험기간약해균교경，표현위식주왜화，생장감만，협편략변소，차무사묘，주고방차분석결과현시여인공제초（ CK）상비차이균불현저；수착시간적연장，약해축점완해。이상규품충기면958대초감련적내수성흔차，분시0.25％화0.50％초감련용액적면화약해증상표현위식주왜화，생장완만，협편변소、고황、갈화간고，병차약해정도수착시간적연장이가중，약해급별화수해지수균고우10-6001，주고방차분석결과현시여인공제초（ CK）상비차이균체도료겁현저수평，시약후35 d부분면화출현정주고사。
In order to define the tolerance of genetically modified cotton 10-6001 resistance to glyphosate, choose transgenic glyphosate resistant cotton material 106-001 and conventional cotton variety Jimian 958 ( CK ) as test materials, spraying on the leaves with different concentrations of 0 ( CK, no spraying herbicide, artificial weeding) , 0.25% and 0.50% glyphosate solution when cotton seedling stage of 6 true leaves, the plant height, level of the adr, suffer index, death rate were investigated respectively after spraying herbicide 0 (afternoon), 1, 7, 14 and 21 d, the death of the cotton seedling situation was investigated after spraying herbicide 35 d, and the response of 2 cotton varieties to different concentrations of glyphosate was analyzed , the analysis of variance on plant height was made.The results showed that the cotton seedling stage of 6 true leaves spraying glyphosate, the tolerance of genetically modified cotton 10-6001 to glyphosate was better, the adr of cotton sprayed 0.25% and 0.50% of glyphosate solution was lighter during the whole test, characterized by plant dwarf, growth slow, leaf blade slightly smaller and on death after spraying 35 d.Variance analysis showed that there was no significant difference on plant height with artificial weeding.The adr gradually ease with the extension of time.While glyphosate tolerance of conventional cotton Jimian 958 pairs was very poor, phytotoxicity symptoms of cotton sprayed 0.25%and 0.50% of glyphosate solution were plant dwarf, growth slow, leaves small and yellow and the adr was increased with the extension of time.The level of the adr and the indexes were all higher than that of 10-6001, the plant height difference was very significant with artificial weeding, some cotton died gradually after spraying 35 d.