中国男科学杂志
中國男科學雜誌
중국남과학잡지
CHINESE JOURNAL OF ANDROLOGY
2013年
11期
8-11
,共4页
睾酮%塞尔托利细胞%氧化应激%细胞凋亡%半胱氨酸天冬氨酸蛋白酶3
睪酮%塞爾託利細胞%氧化應激%細胞凋亡%半胱氨痠天鼕氨痠蛋白酶3
고동%새이탁리세포%양화응격%세포조망%반광안산천동안산단백매3
testosterone%sertoli Cells%oxidative stress%apoptosis%caspase 3
目的：探讨睾酮对睾丸支持细胞TM4氧化应激损伤和细胞凋亡的影响。方法在支持细胞系TM4细胞培养液内分别加入H2O2和睾酮，分为空白组、模型组、低、高剂量睾酮组。在作用24 h后，用MTT法检测吸光度值，用硫代巴比妥酸比色法、黄嘌呤氧化酶法测定SOD活性及MDA含量，用流式细胞仪测定细胞凋亡率和存活率，用实时PCR检测caspase-3 mRNA的表达。结果睾酮低、高剂量组的吸光值由模型组的（0.33±0.05）分别增加至（0.42±0.05）、（0.46±0.07）；SOD活性由（9.01±0.48）U/ml分别增加至（11.02±1.15）U/ml、（12.51±0.98）U/ml；MDA含量由（6.41±0.62）mol/L分别降低至（5.37±0.47）mol/L、（4.28±0.44）mol/L；细胞的凋亡率由（20.10±1.32）%分别降低至（16.13±0.49）%、（10.63±2.29）%；存活率由（68.40±1.00）%分别增加至（73.63±2.94）、（76.87±6.58）；caspase-3 mRNA的表达量由（2.85±0.09）分别降低为（2.34±0.27）、（1.85±0.11）。结论睾酮能改善睾丸支持细胞的氧化应激损伤，抑制细胞凋亡。
目的：探討睪酮對睪汍支持細胞TM4氧化應激損傷和細胞凋亡的影響。方法在支持細胞繫TM4細胞培養液內分彆加入H2O2和睪酮，分為空白組、模型組、低、高劑量睪酮組。在作用24 h後，用MTT法檢測吸光度值，用硫代巴比妥痠比色法、黃嘌呤氧化酶法測定SOD活性及MDA含量，用流式細胞儀測定細胞凋亡率和存活率，用實時PCR檢測caspase-3 mRNA的錶達。結果睪酮低、高劑量組的吸光值由模型組的（0.33±0.05）分彆增加至（0.42±0.05）、（0.46±0.07）；SOD活性由（9.01±0.48）U/ml分彆增加至（11.02±1.15）U/ml、（12.51±0.98）U/ml；MDA含量由（6.41±0.62）mol/L分彆降低至（5.37±0.47）mol/L、（4.28±0.44）mol/L；細胞的凋亡率由（20.10±1.32）%分彆降低至（16.13±0.49）%、（10.63±2.29）%；存活率由（68.40±1.00）%分彆增加至（73.63±2.94）、（76.87±6.58）；caspase-3 mRNA的錶達量由（2.85±0.09）分彆降低為（2.34±0.27）、（1.85±0.11）。結論睪酮能改善睪汍支持細胞的氧化應激損傷，抑製細胞凋亡。
목적：탐토고동대고환지지세포TM4양화응격손상화세포조망적영향。방법재지지세포계TM4세포배양액내분별가입H2O2화고동，분위공백조、모형조、저、고제량고동조。재작용24 h후，용MTT법검측흡광도치，용류대파비타산비색법、황표령양화매법측정SOD활성급MDA함량，용류식세포의측정세포조망솔화존활솔，용실시PCR검측caspase-3 mRNA적표체。결과고동저、고제량조적흡광치유모형조적（0.33±0.05）분별증가지（0.42±0.05）、（0.46±0.07）；SOD활성유（9.01±0.48）U/ml분별증가지（11.02±1.15）U/ml、（12.51±0.98）U/ml；MDA함량유（6.41±0.62）mol/L분별강저지（5.37±0.47）mol/L、（4.28±0.44）mol/L；세포적조망솔유（20.10±1.32）%분별강저지（16.13±0.49）%、（10.63±2.29）%；존활솔유（68.40±1.00）%분별증가지（73.63±2.94）、（76.87±6.58）；caspase-3 mRNA적표체량유（2.85±0.09）분별강저위（2.34±0.27）、（1.85±0.11）。결론고동능개선고환지지세포적양화응격손상，억제세포조망。
Objective To investigate the effect of testosterone on oxidative stress and apoptosis of TM4 Sertoli cells. Methods H2O2 and testosterone were added into medium to stimulate TM4 cells. Cells were divided in the control group, the model group, low and high dose of testosterone group. After 24 h stimulation, proliferation of the cells were detected by MTT, the SOD activity and MDA content in cells were measured by thiobarbituric acid and xanthine oxidase method respectively, the apoptosis and survival rates of cells were determined by flow cytometry, the caspase-3 mRNA expression by real time-PCR. Results The absorbance value of TM4 cells increased from 0.33±0.05 of the model group to 0.42±0.05, 0.46 ±0.07 of the low and high dose of testosterone group. The SOD activity increased from 9.01±0.48 U/ml to 11.02±1.15 U/ml, 12.51±0.98 U/ml respectively. The MDA content decreased from 6.41±0.62 mol/L to 5.37±0.47 mol/L, 4.28±0.44 mol/L respectively. The rate of apoptosis decreased from 20.10±1.32%to 16.13±0.49%, 10.63±2.29%respectively. The rate of cell survival increased from 68.40±1.00%to 73.63±2.94%, 76.87±6.58%respectively. The expression of caspase-3 mRNA decreased from 2.85±0.09 to 2.34±0.27, 1.85±0.11 respectively. Conclusion Testosterone may improve the cells injuried by oxidative stress and inhibit the apoptosis of TM4 cells.
