CAJ | 학술논문

目的：完善通塞益脑口服液的质量标准,为生产质量提供保障。方法：采用TLC法对制剂中的延胡索、川芎、石菖蒲三味药材进行定性鉴别；采用HPLC法对制剂中的总阿魏酸进行含量测定,色谱条件：色谱柱为Lichrospher-C18柱(250 mm ×4.6 mm,5μm),流动相为甲醇：0.1%磷酸(30∶70),流速为1.0 ml·min-1,柱温为30℃,检测波长为321 nm,进样量20μl。结果：TLC鉴别色谱斑点清晰,阴性无干扰；阿魏酸在2.24~35.84μg·ml-1范围内有良好的线性关系,r=0.9999,平均回收率为102.54%,RSD=0.85%(n=6)。结论：该方法准确易行,便于质量控制。
목적：완선통새익뇌구복액적질량표준,위생산질량제공보장。방법：채용TLC법대제제중적연호색、천궁、석창포삼미약재진행정성감별；채용HPLC법대제제중적총아위산진행함량측정,색보조건：색보주위Lichrospher-C18주(250 mm ×4.6 mm,5μm),류동상위갑순：0.1%린산(30∶70),류속위1.0 ml·min-1,주온위30℃,검측파장위321 nm,진양량20μl。결과：TLC감별색보반점청석,음성무간우；아위산재2.24~35.84μg·ml-1범위내유량호적선성관계,r=0.9999,평균회수솔위102.54%,RSD=0.85%(n=6)。결론：해방법준학역행,편우질량공제。
Objective:To improve the quality standard of Tongsai Yinao oral liquids for the quality control. Methods: TLC was used for the qualitative identification of Rhizoma corydails, Rhizoma chuanxiong and Rhizoma acori tatarinowii. HPLC was used to sim-ultaneously determine the content of total ferulic acid in the oral liquids. The determination was performed on a Lichrospher-C18 (250 mm×4.6 mm,5 μm)column with the mobile phase of methanol-0.1% H3PO4(30∶70). The flow rate was 1.0 ml·min-1,the col-umn temperature was at 30℃, the detection wavelength was 321nm and the injection volume was 20μl. Results:The spots on the TLC plates were clear without the interference of negative control. The linear range of ferulic acid was obtained between 2. 24 and 35. 84 μg ·ml-1(r=0. 999 9), and the average recovery was 102. 54%(RSD=0. 85%, n=6). Conclusion:The improved method is accu-rate and feasible. It can be used very conveniently in the quality control.