CAJ | 학술논문

背景与目的：Aurora-A属于丝氨酸和苏氨酸蛋白激酶家族，其异常表达可导致肿瘤发生及其对放射线的抵抗，本研究旨在探讨Aurora-A与细胞放射敏感的相关性。方法：用Aurora-A激酶抑制剂处理Capan-1细胞，检测细胞增殖、锚定非依赖性生长和细胞周期及其周期相关蛋白的变化；处理的细胞同时用γ线照射，然后用流式细胞术检测细胞凋亡。结果：Aurora-A激酶抑制剂处理后，Capan-1细胞生长速度减慢，克隆形成率降低；Aurora-A抑制可引起细胞周期G1/S以及G2/M期转化阻滞，Cyclin D1、CDK2和CDK6表达下降。Aurora-A激酶抑制剂处理后可以提高放射线照射诱导的细胞凋亡率。结论：Aurora-A激酶抑制剂可抑制胰腺癌细胞增殖，阻滞细胞周期，促进细胞对放射线照射的敏感性。因此，Aurora-A可能是胰腺癌放射治疗增敏的靶向分子之一。
배경여목적：Aurora-A속우사안산화소안산단백격매가족，기이상표체가도치종류발생급기대방사선적저항，본연구지재탐토Aurora-A여세포방사민감적상관성。방법：용Aurora-A격매억제제처리Capan-1세포，검측세포증식、묘정비의뢰성생장화세포주기급기주기상관단백적변화；처리적세포동시용γ선조사，연후용류식세포술검측세포조망。결과：Aurora-A격매억제제처리후，Capan-1세포생장속도감만，극륭형성솔강저；Aurora-A억제가인기세포주기G1/S이급G2/M기전화조체，Cyclin D1、CDK2화CDK6표체하강。Aurora-A격매억제제처리후가이제고방사선조사유도적세포조망솔。결론：Aurora-A격매억제제가억제이선암세포증식，조체세포주기，촉진세포대방사선조사적민감성。인차，Aurora-A가능시이선암방사치료증민적파향분자지일。
Background and purpose:Aurora-A is a member of serine/threonine kinase family. The abnormal expression of Aurora-A induces tumorigenesis and radioresistance. This study was aimed to investigate the association of Aurora-A with radioresistance. Methods: Capan-1 cells were treated with Aurora-A kinase inhibitor, and then used to test cell proliferation, anchorage independent assay, cell cycle, and cell cycle regulatory proteins. Treated cells were also used to detect cell apoptosis afterγ-irradiation. Results:Cell growth and colony number in soft agar were decreased after treatment with Aurora-A inhibitor. Treatment of cells with Aurora-A inhibitor also down-regulated the expression of Cyclin D1, CDK2 and CDK6 to induce cell cycle arrest at G1/S and G2/M phases, but promoted cell apoptosis afterγ-irradiation. Conclusion:Treatment of pancreatic cancer cells with Aurora-A kinase inhibitor blocks cell proliferation and cell cycle progression, and promotes sensitivity of cells to radiation. Thus, Aurora-A may be used as one of therapeutic targets to increase the sensitivity of pancreatic cancer radiotherapy.