CAJ | 학술논문

目的：观察2-（2-苯并呋喃基）-2-咪唑啉[2-（-2- benzofuranyl）-2- imidazoline，2- BFI]对实验性自身免疫性脑脊髓炎（EAE）大鼠中枢神经系统内小胶质细胞及MCP-1的影响，并探讨其保护EAE大鼠的作用机制。方法50只雌性SD大鼠随机分为EAE组、2- BFI低剂量组、2- BFI中剂量组、2- BFI高剂量组及对照组，每组10只，采用豚鼠脊髓匀浆免疫诱导SD大鼠建立EAE模型，观察每组大鼠发病情况并进行临床症状评分；采用HE染色和LFB髓鞘染色观察中枢神经系统的病理变化，对其炎症浸润程度加以评分；用免疫组化法测定脑干中激活的小胶质细胞及MCP-1阳性细胞数量。结果与EAE组比较，大、中、小剂量2- BFI干预组大鼠EAE发病率下降，临床症状减轻，潜伏期延长，中枢神经系统内炎性细胞浸润减少，2- BFI中剂量组在EAE临床症状和病理学改变方面差异有统计学意义（P＜0.05）；免疫组化结果显示，与EAE组比较，2- BFI中剂量组的活化的小胶质细胞数及MCP-1阳性细胞数均减少（P＜0.05）。结论中剂量2- BFI对EAE大鼠具有神经保护作用，其作用机制可能与2- BFI抑制小胶质细胞的激活，减少MCP-1的表达有关。
목적：관찰2-（2-분병부남기）-2-미서람[2-（-2- benzofuranyl）-2- imidazoline，2- BFI]대실험성자신면역성뇌척수염（EAE）대서중추신경계통내소효질세포급MCP-1적영향，병탐토기보호EAE대서적작용궤제。방법50지자성SD대서수궤분위EAE조、2- BFI저제량조、2- BFI중제량조、2- BFI고제량조급대조조，매조10지，채용돈서척수균장면역유도SD대서건립EAE모형，관찰매조대서발병정황병진행림상증상평분；채용HE염색화LFB수초염색관찰중추신경계통적병리변화，대기염증침윤정도가이평분；용면역조화법측정뇌간중격활적소효질세포급MCP-1양성세포수량。결과여EAE조비교，대、중、소제량2- BFI간예조대서EAE발병솔하강，림상증상감경，잠복기연장，중추신경계통내염성세포침윤감소，2- BFI중제량조재EAE림상증상화병이학개변방면차이유통계학의의（P＜0.05）；면역조화결과현시，여EAE조비교，2- BFI중제량조적활화적소효질세포수급MCP-1양성세포수균감소（P＜0.05）。결론중제량2- BFI대EAE대서구유신경보호작용，기작용궤제가능여2- BFI억제소효질세포적격활，감소MCP-1적표체유관。
Objective To investigate the effects of 2- (- 2- benzofuranyl)- 2- imidazoline (2- BFI) on microglia activation and MCP- 1 expression in central nervous system (CNS) of rat with experimental autoimmune encephalomyelitis (EAE). Methods Fifty female Sprague- Dawley(SD) rats were randomly divided into five groups:control group(n=10), EAE model group(n=10), low dose (1.5mg/kg ) 2- BFI group (n=10), intermediate dose (3 mg/kg) 2- BFI group (n=10) and high dose (6mg/kg) 2- BFI group (n=10). The model of EAE was induced by injection of guinea pigs spinal cord homogenate (GPSCH)in SD rats. The severity of EAE was scored according to the signs and symptoms. The pathological changes were observed with Hematoxylin- eosin stain-ing and Luxol Fast blue dyeing, then the degree of inflammatory infiltration was evaluated. The changes of activated microglia and MCP- 1 positive cells in brainstem were counted by immunohistochemistry. Results Compared with EAE group, rats in inter-mediate dose 2- BFI treatment groups had lower incidence of disease, prolonged latency, decreased CNS inflammation and de-myelination (P<0.05). Immunohistochemical results showed that the numbers of activated microglia and MCP- 1 positive cells were significantly reduced in intermediate dose 2- BFI group compared with the EAE group (P<0.05). Conclusion 2- BFI at in-termediate dose (3mg/kg) has a beneficial neuroprotective effect on EAE, which may be related to inhibition of microglia activation and reduction of MCP- 1 expression.