中华老年多器官疾病杂志
中華老年多器官疾病雜誌
중화노년다기관질병잡지
CHINESE JOURNAL OF MULTIPLE ORGAN DISEASES IN THE ELDERLY
2014年
1期
54-58
,共5页
陈弘群%杨海华%何悦%曲毅
陳弘群%楊海華%何悅%麯毅
진홍군%양해화%하열%곡의
二十二碳六烯酸%肺疾病,慢性阻塞性%转录因子-RelA
二十二碳六烯痠%肺疾病,慢性阻塞性%轉錄因子-RelA
이십이탄륙희산%폐질병,만성조새성%전록인자-RelA
docosahexaenoic acid%pulmonary disease,chronic obstructive%transcription factor RelA
目的：探讨二十二碳六烯酸（DHA）在慢性阻塞性肺疾病（COPD）中的抗炎机制。方法选取2011年12月至2012年12月在上海市徐汇区中心医院门诊就诊的患者40例，其中COPD稳定期患者20例为A组，COPD发作期患者20例为B组，分离外周静脉血中性粒细胞体外培养，用不同浓度的DHA（10，50和100μmol/L）处理0～72h（0，24，48和72h），收取培养液上清测定炎症因子白细胞介素（IL）-6，IL-8和肿瘤坏死因子α（TNF-α）水平，收取细胞蛋白做Western印迹法检测核因子κB（NF-κB）p65的表达。结果 DHA作用前B组炎症因子水平显著高于A组。DHA作用后，两组炎症因子水平都下降，并呈现浓度和时间依赖效应，B组下降趋势大于A组，高浓度DHA作用72h后，A组和B组炎症因子水平趋于一致。NF-κB p65的表达水平随DHA浓度和时间变化呈现下降趋势（P＜0.05）。结论 DHA可能通过抑制中性粒细胞的NF-κB p65蛋白的表达，显著性地降低了中性粒细胞分泌炎症因子IL-6、IL-8、TNF-α的水平，为DHA用于临床治疗COPD提供了理论依据。
目的：探討二十二碳六烯痠（DHA）在慢性阻塞性肺疾病（COPD）中的抗炎機製。方法選取2011年12月至2012年12月在上海市徐彙區中心醫院門診就診的患者40例，其中COPD穩定期患者20例為A組，COPD髮作期患者20例為B組，分離外週靜脈血中性粒細胞體外培養，用不同濃度的DHA（10，50和100μmol/L）處理0～72h（0，24，48和72h），收取培養液上清測定炎癥因子白細胞介素（IL）-6，IL-8和腫瘤壞死因子α（TNF-α）水平，收取細胞蛋白做Western印跡法檢測覈因子κB（NF-κB）p65的錶達。結果 DHA作用前B組炎癥因子水平顯著高于A組。DHA作用後，兩組炎癥因子水平都下降，併呈現濃度和時間依賴效應，B組下降趨勢大于A組，高濃度DHA作用72h後，A組和B組炎癥因子水平趨于一緻。NF-κB p65的錶達水平隨DHA濃度和時間變化呈現下降趨勢（P＜0.05）。結論 DHA可能通過抑製中性粒細胞的NF-κB p65蛋白的錶達，顯著性地降低瞭中性粒細胞分泌炎癥因子IL-6、IL-8、TNF-α的水平，為DHA用于臨床治療COPD提供瞭理論依據。
목적：탐토이십이탄륙희산（DHA）재만성조새성폐질병（COPD）중적항염궤제。방법선취2011년12월지2012년12월재상해시서회구중심의원문진취진적환자40례，기중COPD은정기환자20례위A조，COPD발작기환자20례위B조，분리외주정맥혈중성립세포체외배양，용불동농도적DHA（10，50화100μmol/L）처리0～72h（0，24，48화72h），수취배양액상청측정염증인자백세포개소（IL）-6，IL-8화종류배사인자α（TNF-α）수평，수취세포단백주Western인적법검측핵인자κB（NF-κB）p65적표체。결과 DHA작용전B조염증인자수평현저고우A조。DHA작용후，량조염증인자수평도하강，병정현농도화시간의뢰효응，B조하강추세대우A조，고농도DHA작용72h후，A조화B조염증인자수평추우일치。NF-κB p65적표체수평수DHA농도화시간변화정현하강추세（P＜0.05）。결론 DHA가능통과억제중성립세포적NF-κB p65단백적표체，현저성지강저료중성립세포분비염증인자IL-6、IL-8、TNF-α적수평，위DHA용우림상치료COPD제공료이론의거。
Objective To investigate the anti-inflammatory effect and mechanism of docosahexaenoic acid (DHA) in patients with chronic obstructive pulmonary disease (COPD). Methods A total of 40 COPD outpatients in our department from December 2011 to December 2012 were recruited in this study. Twenty cases of them in stable period were assigned as group A, and the left 20 cases in exacerbation period were assigned into group B. Their peripheral blood neutrophils were isolated and cultivated. Then the cells were treated with DHA at different concentrations (10, 50 and 100μmol/L) for 0, 24, 48 and 72h, respectively. The levels of the inflammatory cytokines IL-6, IL-8, and TNF-αin culture supernatants were measured. The expression level of NF-κB p65 protein was detected with Western blotting. Results Before DHA treatment, the levels of inflammatory factors were significantly higher in group B than in group A. After DHA treatment, the levels dropped directly in a concentration- and time-dependent manner in both groups, but the downtrend was stronger in group B than in group A. After treated with high dosed DHA for 72h, the inflammatory cytokines levels in group A were not significantly different with those in group B. NF-κB p65 protein expression was decreased in a dose-and time-dependent manner (P<0.05). Conclusions DHA significantly reduces the levels of inflammatory cytokines IL-6, IL-8, and TNF-α through inhibiting NF-κB p65 protein expression in neutrophils, providing the theoretical basis for DHA in clinical treatment of COPD.
