CAJ | 학술논문

大弹涂鱼Boleophthalmus pectinirostris间断分布于西太平洋海岸带东亚与马来西亚马六甲海峡,但马来西亚种群的分类地位尚存争议。研究使用线粒体ND5基因序列(718 bp)与核位点Rag1基因序列(1395 bp)对西北太平洋海岸带11个地点的45尾大弹涂鱼属鱼类进行系统发育关系重建,结果表明大弹涂鱼包括东亚与马来西亚两个单系群,两者形成姊妹群关系。GMYC分析、*BEAST物种树支持大弹涂鱼东亚谱系和马来西亚谱系是不同种。分子测定年龄分析表明大弹涂鱼东亚谱系与马来西亚谱系之间的分化时间为2.73百万年。因此,西北太平洋海岸带大弹涂鱼是复合体,包括两个物种：东亚种群是大弹涂鱼Boleophthalmus pectinirostris sensu stricto,而马来西亚种群是隐存种Boleophthalmus sp.。大弹涂鱼与隐存种之间的物种分化可能是晚上新世冰期海平面下降产生的地理隔离以及间冰期洋流对基因交流的阻碍两方面相互作用的结果。
대탄도어Boleophthalmus pectinirostris간단분포우서태평양해안대동아여마래서아마륙갑해협,단마래서아충군적분류지위상존쟁의。연구사용선립체ND5기인서렬(718 bp)여핵위점Rag1기인서렬(1395 bp)대서북태평양해안대11개지점적45미대탄도어속어류진행계통발육관계중건,결과표명대탄도어포괄동아여마래서아량개단계군,량자형성자매군관계。GMYC분석、*BEAST물충수지지대탄도어동아보계화마래서아보계시불동충。분자측정년령분석표명대탄도어동아보계여마래서아보계지간적분화시간위2.73백만년。인차,서북태평양해안대대탄도어시복합체,포괄량개물충：동아충군시대탄도어Boleophthalmus pectinirostris sensu stricto,이마래서아충군시은존충Boleophthalmus sp.。대탄도어여은존충지간적물충분화가능시만상신세빙기해평면하강산생적지리격리이급간빙기양류대기인교류적조애량방면상호작용적결과。
The species range ofBoleophthalmus pectinirostrissensu lato includes two disjunctive areas, i.e., East Asia and Strait of Malacca in Malaysia along the northwestern Pacific coast. However, the species status of Malaysian populations remains disputed. MitochondrialND5 gene (718 bp) and nuclearRag1 gene (1395 bp) were used to reconstruct phyloge-netic relationships among Boleophthalmus pectinirostris fishes by sampling 45 specimens from 11 locations in the north-western Pacific. The results showed thatBoleophthalmus pectinirostrisfishes could be divided into two major mono-phyletic groups, i.e., East Asian lineage and Malaysian lineage, and which together formed the sister-group relationship. Species delineation using the analyses of GMYC and *Beast species tree supports that East Asian lineage and Malasian lineage ofBoleophthalmus pectinirostrissensu lato should be placed into two different species. Molecular dating revealed that the divergence time between East Asian lineage and Malaysian lineage ofBoleophthalmus pectinirostrissensu lato was 2.73 Ma. We concluded thatBoleophthalmus pectinirostrissensu lato was a complex, including two species. The East Asian populations isBoleophthalmus pectinirostris sensu stricto,and the Malaysian populations is a cryptic species (Bo-leophthalmus sp.). Our findings suggested that species split betweenBoleophthalmus pectinirostris sensu strictoandBo-leophthalmus sp. was attributed to geographical isolation during lowing sea levels of ice ages and the barrier of gene flow induced by ocean currents during interglacial period in the late Pliocene.