CAJ | 학술논문

以酪蛋白和明胶为蛋白源,豆油为脂肪源配制团头鲂幼鱼基础饲料,制成维生素C水平为0.2、33.4、65.8、133.7、251.5和501.5 mg/kg的6种等氮等能的试验饲料。对均重为(6.40±0.05) g的团头鲂幼鱼进行为期90d的饲养试验,以血清相关免疫指标、肝脏抗氧化指标、三种HSPs mRNA表达以及抗病原菌能力等指标为依据,研究Vc对团头鲂幼鱼免疫力的影响。试验结果表明,与对照组相比,501.5 mg/kg Vc试验组能显著提高血清补体3(C3)的浓度,133.7 mg/kg Vc试验组能显著提高补体4(C4)的浓度；65.8、133.7和251.5 mg/kg Vc试验组能显著提高肝脏超氧化物歧化酶(SOD)的活性,各Vc试验组均能显著提高肝脏抗超氧阴离子(ASAFR)的活性；133.7和251.7 mg/kg Vc试验组能显著提高肝脏HSP60基因表达水平,251.5 mg/kg Vc试验组能显著提高肝脏 HSP70和 HSP90基因表达水平(P<0.05)；各 Vc 试验组鱼的成活率在感染嗜水气单胞菌后12h、24h均显著高于对照组(P<0.05),其中以251.5 mg/kg Vc试验组效果最佳。在日粮中添加Vc对鱼体血清碱性磷酸酶(ALP)、总蛋白(TP)、白蛋白(Alb)、皮质醇(COR)以及肝脏丙二醛(MDA)的水平无显著影响(P>0.05)。综上所述,在试验条件下, Vc作为免疫刺激剂,其水平为133.7-251.5 mg/kg时能有效地增强团头鲂幼鱼的免疫力。
이락단백화명효위단백원,두유위지방원배제단두방유어기출사료,제성유생소C수평위0.2、33.4、65.8、133.7、251.5화501.5 mg/kg적6충등담등능적시험사료。대균중위(6.40±0.05) g적단두방유어진행위기90d적사양시험,이혈청상관면역지표、간장항양화지표、삼충HSPs mRNA표체이급항병원균능력등지표위의거,연구Vc대단두방유어면역력적영향。시험결과표명,여대조조상비,501.5 mg/kg Vc시험조능현저제고혈청보체3(C3)적농도,133.7 mg/kg Vc시험조능현저제고보체4(C4)적농도；65.8、133.7화251.5 mg/kg Vc시험조능현저제고간장초양화물기화매(SOD)적활성,각Vc시험조균능현저제고간장항초양음리자(ASAFR)적활성；133.7화251.7 mg/kg Vc시험조능현저제고간장HSP60기인표체수평,251.5 mg/kg Vc시험조능현저제고간장 HSP70화 HSP90기인표체수평(P<0.05)；각 Vc 시험조어적성활솔재감염기수기단포균후12h、24h균현저고우대조조(P<0.05),기중이251.5 mg/kg Vc시험조효과최가。재일량중첨가Vc대어체혈청감성린산매(ALP)、총단백(TP)、백단백(Alb)、피질순(COR)이급간장병이철(MDA)적수평무현저영향(P>0.05)。종상소술,재시험조건하, Vc작위면역자격제,기수평위133.7-251.5 mg/kg시능유효지증강단두방유어적면역력。
A 90-day feeding trial was conducted to determine the effects of vitamin C on the non-specific immunity, threeHSPs mRNA expression and disease resistance of juvenile Wuchang bream (Megalobrama amblycephala) [initial mean body weight of (6.40±0.05) g]. The fish was selected and randomly divided into six groups and Vc supplemented at 0.2, 33.4, 65.8, 133.7, 251.5 and 501.5 mg/kg diet, respectively. The fish was fed for 90 day. Compared with the con-trol, the contents of serum C3 of the 501.5 mg/kg Vc group were increased, the contents of serum C4 of the 133.7 mg/kg Vc group were improved; the hepatic SOD activities of 65.8-251.5 mg/kg Vc groups were enhanced and the hepatic ASAFR activities of all treated groups were enhanced; the expression level ofHSP60 mRNA of 133.7 and 251.5 mg/kg Vc groups were improved, the expression levels ofHSP70 andHSP90 mNA of 251.5 mg/kg Vc group were significantly improved (P<0.05); the survival rates in all treated groups were significantly higher than that of the control at 12 h and 24 h after injectingAeromonas hydrophila (P<0.05), and the survival rate of the 251.5 mg/kg Vc group was the highest. Other indices, such as total protein, albumin, serum cortisol and MDA were not significantly different from the control (P>0.05). Therefore, the basal diet supplemented with 133.7-251.5 mg/kg doses of Vc could improve the immunity of juvenile Wuchang bream effectively.