CAJ | 학술논문

目的：比较2010年从广州市分离到的甲型H1 N1流感病毒神经氨酸酶（NA）基因与2009年中国大陆甲型H1 N1流感病毒NA基因的变异情况，为甲型H1 N1流感的监测和防控提供参考资料。方法收集2010年广州市有发热和呼吸道症状患者的咽拭子标本，用甲型H1 N1流感病毒特异性引物进行聚合酶链反应（PCR）检测，扩增分离到的甲型H1 N1流感病毒NA基因片段，测序后与2009年的H1 N1毒株进行比对和进化分析，并用生物信息学方法对耐药位点和糖基化位点进行分析。结果共收集1194份咽拭子标本，检测到甲型流感病毒阳性327份，其中H1 N1流感病毒6株，与2009年分离的甲型H1 N1流感病毒相比，有16个位点发生了有义突变，3个位点和NA活性相关，其中222位氨基酸的变异位于NA活性位点上。结论成功扩增了2010年广州市6株甲型H1 N1流感病毒株NA基因并测序，未发现H275 Y耐药位点的变异。3毒株在NA活性位点222位、228位和425位等氨基酸位点处发生了变异，需继续加强监测。
목적：비교2010년종엄주시분리도적갑형H1 N1류감병독신경안산매（NA）기인여2009년중국대륙갑형H1 N1류감병독NA기인적변이정황，위갑형H1 N1류감적감측화방공제공삼고자료。방법수집2010년엄주시유발열화호흡도증상환자적인식자표본，용갑형H1 N1류감병독특이성인물진행취합매련반응（PCR）검측，확증분리도적갑형H1 N1류감병독NA기인편단，측서후여2009년적H1 N1독주진행비대화진화분석，병용생물신식학방법대내약위점화당기화위점진행분석。결과공수집1194빈인식자표본，검측도갑형류감병독양성327빈，기중H1 N1류감병독6주，여2009년분리적갑형H1 N1류감병독상비，유16개위점발생료유의돌변，3개위점화NA활성상관，기중222위안기산적변이위우NA활성위점상。결론성공확증료2010년엄주시6주갑형H1 N1류감병독주NA기인병측서，미발현H275 Y내약위점적변이。3독주재NA활성위점222위、228위화425위등안기산위점처발생료변이，수계속가강감측。
Objective To compare the variations of neuraminidase (NA)gene of influenza A (H1 N1 )virus from Guangzhou in 201 0 and NA gene of influenza A (H1 N1 )virus from Chinese mainland in 2009,and to provide the reference for the surveillance and prevention of influenza A (H1 N1 )virus.Methods Specimens were collected from patients with febrile respiratory tract symptoms from Guangzhou in 201 0.The specimens were determined with influenza A (H1 N1 )virus specificity primers by polymerase chain reaction (PCR).The fragment of NA gene was amplified. After sequencing,the results were compared with influenza A (H1 N1 )virus in 2009.The drug resistance sites and glycosylation sites were analyzed by biometric software.Results A total of 1 1 94 specimens were collected,and 327 influenza A virus strains were detected,including 6 strains were identified with influenza A (H1 N1 )virus.Compared with influenza A (H1 N1 )virus in 2009,there were 1 6 amino acid sites with variation.There were 3 amino acid sites relating with NA activity.However,the amino acid site 222 located in the NA activity sites.Conclusions NA gene of the 6 strains of influenza A (H1 N1 )virus are amplified and sequenced successfully,and H275 Y drug resistant site is not found.The amino acid site 222,228 and 425 locating in NA activity sites are found with mutations in 3 strains. Therefore,the monitoring should be strengthened.