检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2014年
1期
38-41
,共4页
鲍彩丽%范倩燕%吴迪%汤园园%谈秋雯%张雪莲
鮑綵麗%範倩燕%吳迪%湯園園%談鞦雯%張雪蓮
포채려%범천연%오적%탕완완%담추문%장설련
粪肠球菌%屎肠球菌%耐药性%耐药基因%抗菌药物
糞腸毬菌%屎腸毬菌%耐藥性%耐藥基因%抗菌藥物
분장구균%시장구균%내약성%내약기인%항균약물
Enterococcus faecalis%Enterococcus faecium%Drug resistance%Drug resistance gene%Antibacterial drug
目的：分析安图医院尿路感染患者中段尿中分离出的粪肠球菌和屎肠球菌的耐药性及耐药基因，为临床用药提供参考。方法选取尿路感染患者中段尿中分离出的粪肠球菌（23株）和屎肠球菌（18株），采用API进行菌种鉴定，纸片扩散法进行体外药物敏感性试验，并采用聚合酶链反应（PCR）对其耐药基因进行检测。结果23株粪肠球菌中tetM、ermB、aac（6′）/aph（2′）、ant（6）-Ⅰ和aph（3′）-Ⅲ基因阳性检出率分别为65．2％、82．6％、100．0％、100．0％和100．0％；18株屎肠球菌中tetM、ermB、aac（6′）/aph（2′）、ant（6）-Ⅰ和aph（3′）-Ⅲ基因阳性检出率分别为55．5％、55．5％、100．0％、94．4％和100．0％。粪肠球菌对青霉素、四环素、环丙沙星、左氧氟沙星、红霉素的耐药率＞50．0％，屎肠球菌对青霉素、氨苄西林、环丙沙星、左氧氟沙星、红霉素的耐药率为100．0％。结论临床分离的肠球菌属耐药相关基因携带率很高，肠球菌属对多种抗菌药物耐药率较高。
目的：分析安圖醫院尿路感染患者中段尿中分離齣的糞腸毬菌和屎腸毬菌的耐藥性及耐藥基因，為臨床用藥提供參攷。方法選取尿路感染患者中段尿中分離齣的糞腸毬菌（23株）和屎腸毬菌（18株），採用API進行菌種鑒定，紙片擴散法進行體外藥物敏感性試驗，併採用聚閤酶鏈反應（PCR）對其耐藥基因進行檢測。結果23株糞腸毬菌中tetM、ermB、aac（6′）/aph（2′）、ant（6）-Ⅰ和aph（3′）-Ⅲ基因暘性檢齣率分彆為65．2％、82．6％、100．0％、100．0％和100．0％；18株屎腸毬菌中tetM、ermB、aac（6′）/aph（2′）、ant（6）-Ⅰ和aph（3′）-Ⅲ基因暘性檢齣率分彆為55．5％、55．5％、100．0％、94．4％和100．0％。糞腸毬菌對青黴素、四環素、環丙沙星、左氧氟沙星、紅黴素的耐藥率＞50．0％，屎腸毬菌對青黴素、氨芐西林、環丙沙星、左氧氟沙星、紅黴素的耐藥率為100．0％。結論臨床分離的腸毬菌屬耐藥相關基因攜帶率很高，腸毬菌屬對多種抗菌藥物耐藥率較高。
목적：분석안도의원뇨로감염환자중단뇨중분리출적분장구균화시장구균적내약성급내약기인，위림상용약제공삼고。방법선취뇨로감염환자중단뇨중분리출적분장구균（23주）화시장구균（18주），채용API진행균충감정，지편확산법진행체외약물민감성시험，병채용취합매련반응（PCR）대기내약기인진행검측。결과23주분장구균중tetM、ermB、aac（6′）/aph（2′）、ant（6）-Ⅰ화aph（3′）-Ⅲ기인양성검출솔분별위65．2％、82．6％、100．0％、100．0％화100．0％；18주시장구균중tetM、ermB、aac（6′）/aph（2′）、ant（6）-Ⅰ화aph（3′）-Ⅲ기인양성검출솔분별위55．5％、55．5％、100．0％、94．4％화100．0％。분장구균대청매소、사배소、배병사성、좌양불사성、홍매소적내약솔＞50．0％，시장구균대청매소、안변서림、배병사성、좌양불사성、홍매소적내약솔위100．0％。결론림상분리적장구균속내약상관기인휴대솔흔고，장구균속대다충항균약물내약솔교고。
Objective To analyze the drug resistance and gene of Enterococcus faecalis and Enterococcus faecium isolated from the midstream urine of patients with urinary tract infection in Antu Hospital,and to provide the reference for clinical medication.Methods Enterococcus faecalis (23 isolates)and Enterococcus faecium (1 8 isolates)were isolated and collected from the midstream urine of patients with urinary tract infection.The bacteria were identified by API,and drug sensitivity test was done by Kirby-Bauer disk diffusion method.Drug resistance genes were also detected by polymerase chain reaction(PCR).Results The positive rates of tetM,ermB,aac(6′)/aph(2′),ant(6)-Ⅰ and aph(3′)-Ⅲ genes were 65.2%,82.6%,1 00.0%,1 00.0% and 1 00.0% in the 23 isolates of Enterococcus faecalis. In the 1 8 isolates of Enterococcus faecium,the positive rates of tetM,ermB,aac(6′)/aph(2′),ant(6)-Ⅰ and aph (3′)-Ⅲ genes were 55.5%,55.5%,1 00.0%,94.4% and 1 00.0%.The drug resistance rates of Enterococcus faecalis to panicillin,tetracyline,ciprofloxacin,levofloxacin and erythomycin were >50.0%.The drug resistance rates of Enterococcus faecium to penicillin, ampicillin, ciprofloxacin, levofloxacin and erythomycin were 1 00.0%. Conclusions The positive rate of drug resistance genes is high in Enterococcus isolated from clinic,which has a high multi-drug resistance.
