CAJ | 학술논문

为探讨重金属对贝类早期生活史阶段生理生化指标的影响及可能的毒性机制，以太平洋牡蛎的胚胎和幼虫为材料，研究重金属汞对其早期发育阶段3种与免疫防御等功能相关酶活力的影响。试验结果显示，3μg/L、30μg/L和300μg/L的汞暴露2 h （桑葚胚期）、5 h （原肠期）、8 h （担轮幼虫期）和20 h （D形面盘幼虫期），胚胎和幼虫的酸性磷酸酶和碱性磷酸酶的活力水平均显著低于对照组（ P＜0．01）；3μg/L和30μg/L汞暴露后过氧化物酶的活力也受到显著抑制（ P＜0．01），而300μg/L的汞暴露后的过氧化物酶活力较对照组显著升高（P＜0．01）。对照组这3种酶的活力随发育期而变化，其活力水平值均在桑葚期有一个升高，原肠胚期和担轮幼虫期较低，而D形面盘幼虫期又有所升高。本研究结果表明，重金属对酸性磷酸酶、碱性磷酸酶及过氧化物酶活力的影响可能是其导致双壳贝类胚胎和幼虫毒性的原因之一。
위탐토중금속대패류조기생활사계단생리생화지표적영향급가능적독성궤제，이태평양모려적배태화유충위재료，연구중금속홍대기조기발육계단3충여면역방어등공능상관매활력적영향。시험결과현시，3μg/L、30μg/L화300μg/L적홍폭로2 h （상심배기）、5 h （원장기）、8 h （담륜유충기）화20 h （D형면반유충기），배태화유충적산성린산매화감성린산매적활력수평균현저저우대조조（ P＜0．01）；3μg/L화30μg/L홍폭로후과양화물매적활력야수도현저억제（ P＜0．01），이300μg/L적홍폭로후적과양화물매활력교대조조현저승고（P＜0．01）。대조조저3충매적활력수발육기이변화，기활력수평치균재상심기유일개승고，원장배기화담륜유충기교저，이D형면반유충기우유소승고。본연구결과표명，중금속대산성린산매、감성린산매급과양화물매활력적영향가능시기도치쌍각패류배태화유충독성적원인지일。
The effects of heavy metal Hg on three defense related enzyme activities were investigated in embryos and larvae of Pacific oyster Crassostrea giga to elucidate the effects of heavy metals on physiological and biochemical indexes and toxic mechanism during early developmental stages of bivalves . The results showed that the acid phosphatase (ACP) and alkaline phosphatase (ALP) activities were found to be reduced significantly (P<0 .01) in embryos and larvae exposed to Hg concentrations of 3 μg/L ,30μg/L and 300 μg/L for 2 h (morula stage) ,5 h (gastrula stage) ,8 h (trochophore larval stage) ,and 20 h (D-shaped veliger larval stage) compared with those in control group .The peroxidase (POD) activities of embryos and larvae were also found to be inhibited significantly (P<0 .05) in the groups of 3μg/L and 30μg/L compared with the control group ,while the POD activity were increased in 30 μg/L group (P<0 .01) .In control group ,three enzyme activities were changed with the development of Pacific oyster , with increases in activity in morula stag ,then decrease ,the minimum in gastrula and trochophore stages and increase in veliger larvae .The findings indicate that the toxic effects of metal on embryos and larvae of bivalves might be involved in the changes in ACP ,ALP and POD activities .