CAJ | 학술논문

为查明苯系物对仿刺参体内脂质的过氧化程度的影响，在水温（17±0．5）℃和盐度32±0．5条件下，研究了不同质量浓度和不同时间的苯系物（苯、甲苯、乙苯、二甲苯）暴露对仿刺参[体质量（5．00±0．25）g]体腔液中脂质过氧化作用产物丙二醛含量的影响。试验结果表明，低质量浓度的苯（0．81mg/L）和甲苯（0．47mg/L）对仿刺参体内的丙二醛含量无显著影响。中质量浓度和高质量浓度的苯（4．06、20．32 mg/L ）、甲苯（2．33、11．66 mg/L ）以及三种质量浓度的乙苯（0．35、1．74、8．68 mg/L）、邻二甲苯（0．28、1．38、6．92 mg/L）、间二甲苯（0．22、1．09、5．44 mg/L）、对二甲苯（0．16、0．81、4．05 m g/L ）暴露后，仿刺参体内的丙二醛含量与时间均存在显著的剂量-效应关系，丙二醛含量随着时间的增加而逐渐降低，间二甲苯和对二甲苯对仿刺参体内的丙二醛含量具有极显著的抑制作用。试验结果表明，苯系物能够引起仿刺参体内脂质过氧化损伤，仿刺参体腔液中的丙二醛含量可以作为指示海洋环境中苯系物污染程度的指标。
위사명분계물대방자삼체내지질적과양화정도적영향，재수온（17±0．5）℃화염도32±0．5조건하，연구료불동질량농도화불동시간적분계물（분、갑분、을분、이갑분）폭로대방자삼[체질량（5．00±0．25）g]체강액중지질과양화작용산물병이철함량적영향。시험결과표명，저질량농도적분（0．81mg/L）화갑분（0．47mg/L）대방자삼체내적병이철함량무현저영향。중질량농도화고질량농도적분（4．06、20．32 mg/L ）、갑분（2．33、11．66 mg/L ）이급삼충질량농도적을분（0．35、1．74、8．68 mg/L）、린이갑분（0．28、1．38、6．92 mg/L）、간이갑분（0．22、1．09、5．44 mg/L）、대이갑분（0．16、0．81、4．05 m g/L ）폭로후，방자삼체내적병이철함량여시간균존재현저적제량-효응관계，병이철함량수착시간적증가이축점강저，간이갑분화대이갑분대방자삼체내적병이철함량구유겁현저적억제작용。시험결과표명，분계물능구인기방자삼체내지질과양화손상，방자삼체강액중적병이철함량가이작위지시해양배경중분계물오염정도적지표。
T he effects of various concentrations and exposure time of beneze ,toluene ,ethylbenzene ,and xylene (BTEX) on lipid peroxidation and malonaldehyde (MDA) content were studied in the coelomic fluid of sea cucumber A postichopus j aponicus with body weight of (5 .00 ± 0 .25) g exposed to the BTEX for 0 h ,24 h ,36 h ,48 h ,60 h ,72 h ,84 h and 96 h at water temperature of (17 ± 0 .5) ℃ and a salinity of 32 ± 0 .5 .Results showed that there were no significant changes in MDA content between low concentration benzene (0 .81 mg/L ) and toluene (0 .47 mg/L ) and the control group in the coelomic fluid of the sea cucumber during the experiment .In mid and high concentration of benzene (4 .06 mg/L and 20 .32 mg/L) and toluene (2 .33 mg/L and 11 .66 mg/L) ,and three concentrations of p-xylene (0 .28 mg/L ,1 .38 mg/L and 6 .92 mg/L) ,m-xylene (0 .22 mg/L ,1 .09 mg/L and 5 .44 mg/L) ,o-xylene (0 .16 mg/L ,0 .81 mg/L and 4 .05 mg/L) and ethylbenzen (0 .35 mg/L ,1 .74 mg/L and 8 .68 mg/L) ,however ,the MDA contents were found to be gradually decreased with increase in exposure time ,being inhibited significantly by both p-xylene and m-xylene contents .The findings indicate that BTEX might cause lipid peroxidation injury of the sea cucumber and that the MDA content might be used as biomarkers to evaluate the effects of BTEX on marine environment .