CAJ | 학술논문

以[1-14C]油酸(oleic acid；18：1n-9, OA)为指示剂,研究了不同饲料油源饲喂下大西洋鲑肝细胞膜脂肪酸组成受到改变时该细胞对OA吸收的状况,以探讨植物油(Vegetable oil, VO)替代鱼油(Fish oil, FO)对大西洋鲑肝细胞脂肪酸跨膜吸收的影响,为大西洋鲑饲料中植物油替代鱼油的可行性提供理论依据。试验先以鱼油和大豆油为油源配制两种全价配合饲料,分别饲喂大西洋鲑幼鲑5个月,使其产生不同的脂肪酸组成。在饲养结束后,分离并培养试验鱼肝细胞,将细胞与[1-14C]OA及37.5μmol/L OA(1/30, mol/mol,0.3μCi/瓶)共孵育2h,收集并测定细胞内OA放射活性,再计算细胞内OA吸收量[nmol/(h·million cells)]。同时,试验采取RT-PCR方法测定了细胞脂肪酸运送蛋白(Fatty acid transport protein, FATP)、脂肪酸移位蛋白(Fatty acid translocase, FAT/CD36)的基因表达量。结果表明, FO和VO组肝细胞对OA吸收分别为(0.924±0.258)及(0.888±0.179) nmol/(h·million cells),两组间无显著差异(P>0.05)。RT-PCR的检测结果表明,FAT/CD36和FATP基因表达量在 FO 与 VO 两组间均无显著差异(P>0.05)。结果表明,从植物油替代鱼油不影响大西洋鲑肝细胞对长链脂肪酸的跨膜吸收方面来看,大西洋鲑饲料中以植物油替代鱼油具有可行性。
이[1-14C]유산(oleic acid；18：1n-9, OA)위지시제,연구료불동사료유원사위하대서양해간세포막지방산조성수도개변시해세포대OA흡수적상황,이탐토식물유(Vegetable oil, VO)체대어유(Fish oil, FO)대대서양해간세포지방산과막흡수적영향,위대서양해사료중식물유체대어유적가행성제공이론의거。시험선이어유화대두유위유원배제량충전개배합사료,분별사위대서양해유해5개월,사기산생불동적지방산조성。재사양결속후,분리병배양시험어간세포,장세포여[1-14C]OA급37.5μmol/L OA(1/30, mol/mol,0.3μCi/병)공부육2h,수집병측정세포내OA방사활성,재계산세포내OA흡수량[nmol/(h·million cells)]。동시,시험채취RT-PCR방법측정료세포지방산운송단백(Fatty acid transport protein, FATP)、지방산이위단백(Fatty acid translocase, FAT/CD36)적기인표체량。결과표명, FO화VO조간세포대OA흡수분별위(0.924±0.258)급(0.888±0.179) nmol/(h·million cells),량조간무현저차이(P>0.05)。RT-PCR적검측결과표명,FAT/CD36화FATP기인표체량재 FO 여 VO 량조간균무현저차이(P>0.05)。결과표명,종식물유체대어유불영향대서양해간세포대장련지방산적과막흡수방면래간,대서양해사료중이식물유체대어유구유가행성。
Taking [1-14C] OA (oleic acid;18:1n-9) as the indicator, the uptake of OA in Atlantic salmon (Salmo salar L.) hepatocyte with different membrane fatty acid composition induced by dietary fish oil (FO) and vegetable oil (VO) diets was determined to investigate the effect of replacing dietary fish oil with vegetable oil on fatty acid uptake and to pro-vide the probability of replacing dietary fish oil with vegetable oil in Atlantic salmon diet. Atlantic salmon post smolt was fed diets containing either 100% FO or VO for 5 months to produce hepatocytes with typical FO and VO fatty acid composition, then OA uptake in isolated hepatocytes were studied by incubating with [1-14C] OA and 37.5 μmol/L OA (1/30, mol/mol, 0.3 μCi/flask) for 2h and by calculating the radioactivity in the cells. Meanwhile total RNA of the other same batch of FO and VO hepatocytes were extracted and the relative expression of FATP (fatty acid transport protein) and FAT/CD36 (fatty acid translocase) were determined by RT-PCR. The result showed that OA uptake in FO and VO was (0.924±0.258) nmol/(h·million cells) cells and (0.888±0.179) nmol/(h·million cells) respectively and there was no significant different between them (P>0.05). The relative expression ofFATP andFAT/CD36 were not significantly di-fferent between FO and VO hepatocytes too. The results indicate that by the same OA uptake between FO and VO hepatocytes, replacing dietary FO with VO was available in Atlantic salmon diet.