CAJ | 학술논문

鲀实验比较不同盐度处理对暗纹东方(Takifugu obscurus)的生长、非特异性免疫和抗氧化酶活力的影响。实验设置6‰、12‰、18‰、24‰和30‰5个盐度处理组，淡水组设为对照组。分别在实验的第1、第5、第10、第15、第20、第30、第40和第50天进行取样测定，并进行溶菌酶、碱性磷酸酶、超氧化物歧化酶和过氧化氢酶活力测定。当盐度≤18‰时，鱼的生长受影响较小，而在盐度为24‰和30‰的高盐度组中鱼体生长受到较大抑制，并且死亡率随着盐度的增加而增高。经盐度处理后，溶菌酶和碱性磷酸酶活力先逐渐增加，在达到最高峰后又逐渐降低，在24‰和30‰的高盐度组中，最终酶活力显著低于对照组水平。超氧化物歧化酶和过氧化氢酶的活力也呈现出先增加后降低的趋势，最终保持与对照组相似的水平。结果表明，高盐度(24‰和30‰)鲀对暗纹东方的生长、非特异性免疫和抗氧化酶活力均有显著的影响。
돈실험비교불동염도처리대암문동방(Takifugu obscurus)적생장、비특이성면역화항양화매활력적영향。실험설치6‰、12‰、18‰、24‰화30‰5개염도처리조，담수조설위대조조。분별재실험적제1、제5、제10、제15、제20、제30、제40화제50천진행취양측정，병진행용균매、감성린산매、초양화물기화매화과양화경매활력측정。당염도≤18‰시，어적생장수영향교소，이재염도위24‰화30‰적고염도조중어체생장수도교대억제，병차사망솔수착염도적증가이증고。경염도처리후，용균매화감성린산매활력선축점증가，재체도최고봉후우축점강저，재24‰화30‰적고염도조중，최종매활력현저저우대조조수평。초양화물기화매화과양화경매적활력야정현출선증가후강저적추세，최종보지여대조조상사적수평。결과표명，고염도(24‰화30‰)돈대암문동방적생장、비특이성면역화항양화매활력균유현저적영향。
Effects of salinity on the growth, the non-specific immunity and the antioxidant enzyme activities in Obscure pufferTakifugu obscureswere investigated. The salinity of aquaculture water were maintained at 6‰, 12‰, 18‰, 24‰ and 30‰ in four experimental groups, and group of fresh water was used as control. We collected the samples on day 1, 5, 10, 15, 20, 30, 40 and 50 during the experiment, and we examined the activities of lysozyme, alkaline phosphatase (AKP), superoxide dismutase (SOD) and catalase (CAT) in the samples. The salinity did not show significant impact on the growth when the salinity of culture water was below 18‰. However, the growth was inhibited when treated with salinity of 24‰ and 30‰, and the death rate was also elevated with the increase in salinity. The activities of both ly-sozyme and AKP was first enhanced by the increased salinity, but the activities in water with higher salinities (24‰ and 30‰) were significantly lower than that of in control groups. The activities of SOD and CAT also increased after treat-ment, then gradually decreased and the final level was not significantly different from control groups. The results showed that high salinity of 24‰ and 30‰ significantly affected the growth, non-specific immunity and the activities of antioxidant systems in obscure pufferTakifugu obscures.