CAJ | 학술논문

为了解蔗糖合成酶在巴西橡胶树(Hevea brasiliensis)生长和发育过程中的功能，利用RACE技术从巴西橡胶树中克隆了蔗糖合成酶基因，并对基因的表达特征进行了分析。结果表明，从巴西橡胶树中克隆了两个蔗糖合成酶基因(HbSS1和HbSS2)，HbSS1全长2864 bp，编码806个氨基酸；HbSS2全长2815 bp，编码811个氨基酸。两个基因编码的蛋白具有典型的植物蔗糖合成酶结构特征，包含1个磷酸化位点和两个保守的功能域。半定量RT-PCR分析表明，HbSS1和HbSS2在各组织器官中均有表达，其中HbSS1在叶中的表达量最高，HbSS2在树皮中的表达量最高，这说明HbSS1和HbSS2可能参与了各组织的生长和代谢过程，且功能有所分化。
위료해자당합성매재파서상효수(Hevea brasiliensis)생장화발육과정중적공능，이용RACE기술종파서상효수중극륭료자당합성매기인，병대기인적표체특정진행료분석。결과표명，종파서상효수중극륭료량개자당합성매기인(HbSS1화HbSS2)，HbSS1전장2864 bp，편마806개안기산；HbSS2전장2815 bp，편마811개안기산。량개기인편마적단백구유전형적식물자당합성매결구특정，포함1개린산화위점화량개보수적공능역。반정량RT-PCR분석표명，HbSS1화HbSS2재각조직기관중균유표체，기중HbSS1재협중적표체량최고，HbSS2재수피중적표체량최고，저설명HbSS1화HbSS2가능삼여료각조직적생장화대사과정，차공능유소분화。
To investigate the function of sucrose synthase in Hevea brasiliensis growth and development, two genes encoding sucrose synthase, named as HbSS1 and HbSS2, were cloned from H. brasiliensis by RACE-PCR, and their expression patterns were analysed by semi-quantitative RT-PCR. The results showed that the whole length of HbSS1 cDNA was 2864 bp, encoding 806 amino acids;that of HbSS2 cDNA was 2815 bp, encoding 811 amino acids. Both HbSS1 and HbSS2 had typical characteristic of sucrose synthase in plants, containing a conserved phosphorylation site and two conserved domains. The expressions of HbSS1 and HbSS2 were detected in all tissues, their expressions were the highest in leaves and bark, respectively. It was suggested that HbSS1 and HbSS2 might be involved in growth and metabolism of various tissues, and they also had functional differentiation.