CAJ | 학술논문

为探讨水稻(Oryza sativa)组蛋白脱乙酰化酶基因HDA705的功能和表达特性，根据NCBI上登录的水稻HDA705基因(GenBank登录号：AK111861)的序列，克隆了5′端2 kb的启动子片段proHDA705，并构建了proHDA705：GUS表达载体。通过农杆菌介导法转化水稻，并获得了转基因株系。GUS检测结果表明，proHDA705仅在水稻的根、茎、叶及部分颖壳的表皮毛等器官中表达，而在花器官中不表达，这表明HDA705具有组织表达特异性。
위탐토수도(Oryza sativa)조단백탈을선화매기인HDA705적공능화표체특성，근거NCBI상등록적수도HDA705기인(GenBank등록호：AK111861)적서렬，극륭료5′단2 kb적계동자편단proHDA705，병구건료proHDA705：GUS표체재체。통과농간균개도법전화수도，병획득료전기인주계。GUS검측결과표명，proHDA705부재수도적근、경、협급부분영각적표피모등기관중표체，이재화기관중불표체，저표명HDA705구유조직표체특이성。
In order to understand the function and expression character of histone deacetylase gene HDA705 in rice (Oryza sativa), 5′ terminal promoter sequence was cloned with length of 2 kb based on rice HDA705 sequence (GenBank accession No.:AK111861), named proHDA705. The expression vector of proHDA705:GUS was constructed. The proHDA705:GUS vector was transformed to rice by Agrobacterium-mediated assays. GUS staining assays of transgenic rice showed that proHDA705 expressed only in root, stem, leave and some epidermal hair of seed coat, but did not in lfower. It was suggested that HDA705 had tissue speciifc expression in rice.