安徽农学通报
安徽農學通報
안휘농학통보
AUHUI AGRICULTURAL SCIENCE BULLETIN
2014年
8期
151-152,165
,共3页
罗氏沼虾%诺达病毒%三抗体夹心酶联免疫吸附测定%逆转录聚合酶链式反应
囉氏沼蝦%諾達病毒%三抗體夾心酶聯免疫吸附測定%逆轉錄聚閤酶鏈式反應
라씨소하%낙체병독%삼항체협심매련면역흡부측정%역전록취합매련식반응
Macrobrachium Rosenbergii%Nodavirus%Three antibody sandwich ELISA%Reverse transcriptase polymerase chain reaction
分别用三抗体夹心酶联免疫吸附测定、逆转录聚合酶链式反应两种方法,检测20尾采集自广东省肇庆市的典型白肉病症状罗氏沼虾肌肉中的诺达病毒。检测结果是两种方法阳性检出率均为95%。结果表明,两种方法均能有效的应用在实验室检测罗氏沼虾诺达病毒。
分彆用三抗體夾心酶聯免疫吸附測定、逆轉錄聚閤酶鏈式反應兩種方法,檢測20尾採集自廣東省肇慶市的典型白肉病癥狀囉氏沼蝦肌肉中的諾達病毒。檢測結果是兩種方法暘性檢齣率均為95%。結果錶明,兩種方法均能有效的應用在實驗室檢測囉氏沼蝦諾達病毒。
분별용삼항체협심매련면역흡부측정、역전록취합매련식반응량충방법,검측20미채집자광동성조경시적전형백육병증상라씨소하기육중적낙체병독。검측결과시량충방법양성검출솔균위95%。결과표명,량충방법균능유효적응용재실험실검측라씨소하낙체병독。
Three antibodies sandwich ELISA,reverse transcriptase polymerase chain reaction were used re-spectively to detect the Nodavirus from the muscle of 20 Macrobrachium Rosenbergii collected from Zhaoq-ing City,Guangdong Province,which have typical symptoms of white meat disease. The positive rate tested from these two methods was the same: 95%. The results of the comparison showed that both methods are effective on laboratory detection of Macrobrachium Rosenbergii Nodavirus.