CAJ | 학술논문

目的：探讨羊水栓塞（AFE）肺损伤发病机制，探讨转化生长因子-β1（TGF-β1）在 AFE 肺损伤中的作用。方法将20只健康妊娠晚期家兔随机分为对照组（生理盐水组，10只）及羊水组（10只），建立 AFE 动物模型，1 h 后取家兔肺组织制作病理切片观察肺组织病理变化，采用链霉素亲和-生物素-过氧化酶复合物法对肺组织细支气管上皮细胞 TGF-β1蛋白表达进行免疫组织化学染色，采用 MIAS-2000医学图像分析系统进行蛋白半定量分析，以最大灰度值反映 TGF-β1蛋白表达量。结果羊水组肺组织病理切片见肺组织水肿，肺组织 TGF-β1蛋白水平：对照组（5．02±0．76）μg/L ，羊水组（19．85±1．92）μg/L ，羊水组 TGF-β1蛋白水平明显升高（P ＜0．05）。结论AFE 肺损伤与 TGF-β1水平升高密切相关。
목적：탐토양수전새（AFE）폐손상발병궤제，탐토전화생장인자-β1（TGF-β1）재 AFE 폐손상중적작용。방법장20지건강임신만기가토수궤분위대조조（생리염수조，10지）급양수조（10지），건립 AFE 동물모형，1 h 후취가토폐조직제작병리절편관찰폐조직병리변화，채용련매소친화-생물소-과양화매복합물법대폐조직세지기관상피세포 TGF-β1단백표체진행면역조직화학염색，채용 MIAS-2000의학도상분석계통진행단백반정량분석，이최대회도치반영 TGF-β1단백표체량。결과양수조폐조직병리절편견폐조직수종，폐조직 TGF-β1단백수평：대조조（5．02±0．76）μg/L ，양수조（19．85±1．92）μg/L ，양수조 TGF-β1단백수평명현승고（P ＜0．05）。결론AFE 폐손상여 TGF-β1수평승고밀절상관。
Objective To explore the pathogenesis of lung injury caused by amniotic fluid embolism (AFE) and the effects of transforming growth factor-β1 (TGF-β1 ) on AFE caused acute lung injury .Methods 20 healthy late pregnancy rabbits were randomly divided into the control group (10 cases ,injected with normal saline) and the amni-otic group(10 cases ,injected with amniotic fluid) to establish the animal model of AFE .The pulmonary tissue of AFE rabbits was taken out after 1h for preparing the lung tissue sections .The pulmonary tissue pathological changes were observed .The streptavidin-biotin-peroxidase complex(SABC) method was adopted to conduct the immunohistochemi-cal staining on lung bronchiole epithelial cells TGF-β1 protein expression ,and the MIAS-2000 image analysis system was adopted to perform the semi-quantitative analysis of proteins ,with the maximum gray value for reflecting the a-mount of TGF-β1 protein expression .Results Lung tissue edema could be fined in lung tissue biopsy of the amniotic group .The TGF-β1 protein content was(19 .85 ± 1 .92)μg/L in the control group and(5 .02 ± 0 .76)μg/L in the amni-otic group ,the TGF-β1 content of lung tissue in the amniotic group was significantly increased (P< 0 .05) .Conclusion AFE caused acute lung injury is closely related with the TGF-β1 level .