CAJ | 학술논문

目的：比较人基底样乳腺癌和管腔 A 乳腺癌干/祖细胞分化前后的细胞表型变化。方法应用乳腺癌细胞球无血清悬浮培养法获取乳腺癌干/祖细胞；分别以荧光免疫技术和流式细胞术检测基底样乳腺癌和管腔 A乳腺癌干/祖细胞分化前后细胞角蛋白 CK14、CK18、CK19和 CD44、CD24的表达情况；分析原代细胞内 CD44＋CD24-细胞水平与克隆形成率的关系。结果基底样乳腺癌干/祖细胞表达 CK19＋/CK14＋，不表达 CK18-，经血清诱导分化后，管腔上皮标志 CK18和肌上皮标志 CK14呈阳性表达；管腔 A 乳腺癌干/祖细胞呈 CK18＋/CK19＋/CK14-表型，诱导分化后管腔上皮标志 CK18和 CK19表达，而肌上皮细胞标志 CK14则不表达；CD24在基底样乳腺癌表达极低或无表达，但在管腔 A 乳腺癌高表达。基底样乳腺癌原代细胞中水平较高的是 CD44-/CD24-/Low 细胞，含 CD44＋/CD24-/Low 细胞（2．52±0．58）％，而管腔 A 乳腺癌 CD44-/CD24＋细胞所占的比例最高，但不含CD44＋/CD24-/Low 细胞或水平极低。结论基底样乳腺癌和管腔 A 乳腺癌干/祖细胞有不同的细胞表型，二者的分化能力和分化方向也不同，可能起源于不同的乳腺上皮细胞。
목적：비교인기저양유선암화관강 A 유선암간/조세포분화전후적세포표형변화。방법응용유선암세포구무혈청현부배양법획취유선암간/조세포；분별이형광면역기술화류식세포술검측기저양유선암화관강 A유선암간/조세포분화전후세포각단백 CK14、CK18、CK19화 CD44、CD24적표체정황；분석원대세포내 CD44＋CD24-세포수평여극륭형성솔적관계。결과기저양유선암간/조세포표체 CK19＋/CK14＋，불표체 CK18-，경혈청유도분화후，관강상피표지 CK18화기상피표지 CK14정양성표체；관강 A 유선암간/조세포정 CK18＋/CK19＋/CK14-표형，유도분화후관강상피표지 CK18화 CK19표체，이기상피세포표지 CK14칙불표체；CD24재기저양유선암표체겁저혹무표체，단재관강 A 유선암고표체。기저양유선암원대세포중수평교고적시 CD44-/CD24-/Low 세포，함 CD44＋/CD24-/Low 세포（2．52±0．58）％，이관강 A 유선암 CD44-/CD24＋세포소점적비례최고，단불함CD44＋/CD24-/Low 세포혹수평겁저。결론기저양유선암화관강 A 유선암간/조세포유불동적세포표형，이자적분화능력화분화방향야불동，가능기원우불동적유선상피세포。
Objective To compare the phenotypic change of stem /progenitor cells from basal-like breast cancer and luminal-a breast cancer before and after differentiation .Methods The breast cancer stem /progenitor cells were obtained by the breast cancer mammosphere (MS ) serum-free Maitland culture ;the expression of CK14 ,CK18 , CK19 ,CD44 and CD24 before stem/progenitor cells differentiation in basal-like breast cancer and luminal-a breast cancer were detected by the immunofluorescence and the flow cytometry ;the relation berween the content of CD44 , CD24 cells in the primary cells with the cloning efficiency (Msfe) were analyzed .Results The stem /progenitor cells of basal-like breast cancer showed the CK19 + /CK14 + CK18 - phenotype ,after the induced differentiation ,expressed the luminal epithelium marker CK18 and myoepithelium marker CK14 .The stem/progenitor cell phenotype of basal-like breast cancer and luminal-a breast cancer showed CK18 + /CK19 + /CK14 - ,after the induced differentiation ,ex-pressed the luminal epithelium marker CK18 and CK19 ,did not express CK14 ;CD24 had the extreme low expression or no expression in basal-like breast cancer ,but had high expression in luminal a breast cancer .CD44 - /CD24 - /Low cells had hgher content in basal-like breast cancer primary cells ,containing CD44 - /CD24 - /Low cells for (2 .52 ± 0 .58)% ,but luminal a breast cancer had highest content of CD44 - /CD24 + cells ,did nod contain CD44 + /CD24 - /Low cells or their content was extremely low .Conclusion The stem/progenitor cells of basal-like breast cancers and lu-minal-A breast cancer exhibit different phenotypes .Their differentiation ability and direction are different ,which im-plied that they could be originated from different normal mammary epithelial cells .