CAJ | 학술논문

本文以紫玉兰为材料，采用超声波结合酶法提取紫玉兰色素，采用单因素和正交实验研究不同酶用量、酶解时间、酶解温度、乙醇体积分数、超声时间、料液比等条件对色素提取的影响，并确定最佳的提取工艺。同时研究光照、pH值、不同浓度NaCl、碳水化合物和金属离子对紫玉兰色素稳定性的影响。结果表明：紫玉兰色素提取的最佳工艺参数为纤维素酶用量4mg/g （花瓣湿重），按1∶15的料液比加入60％的乙醇溶液，在50℃酶解80min ，超声辅助提取10min。该色素对强碱和光照不稳定，NaCl和碳水化合物对其有护色作用，Fe3＋和Cu2＋离子对色素稳定性影响较大。
본문이자옥란위재료，채용초성파결합매법제취자옥란색소，채용단인소화정교실험연구불동매용량、매해시간、매해온도、을순체적분수、초성시간、료액비등조건대색소제취적영향，병학정최가적제취공예。동시연구광조、pH치、불동농도NaCl、탄수화합물화금속리자대자옥란색소은정성적영향。결과표명：자옥란색소제취적최가공예삼수위섬유소매용량4mg/g （화판습중），안1∶15적료액비가입60％적을순용액，재50℃매해80min ，초성보조제취10min。해색소대강감화광조불은정，NaCl화탄수화합물대기유호색작용，Fe3＋화Cu2＋리자대색소은정성영향교대。
In this paper ,the pigment was extracted by the ultrasonic -assisted enzymatic method .The ex-traction conditions were optimized through the single factor and orthogonal tests .The effects of the dosage of enzyme ,the concentration of extracting agent ,enzymolysis time ,temperature ,ultrasound time and the material/liquid ratio on pigment were studied in this paper .The optimum conditions were as follows :en-zyme dosage 4mg/g (petal weight) ,60% ethanol ,soaking for 80min at 50℃ with the material/liquid ratio of 1 :15 ,ultrasonic extraction of 15min .The stability experimental results showed that the pigment from magnolia liliiflora was applicable in acidic conditions .The NaCl and some carbohydrates had the effect of color-protecting .But the pigment was unstable against light ,alkaline environment and some metal ions such as Fe3+ and Cu2+ .