中华围产医学杂志
中華圍產醫學雜誌
중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2013年
8期
479-484
,共6页
张琳琳%李莹%杨小倩%张展
張琳琳%李瑩%楊小倩%張展
장림림%리형%양소천%장전
先兆子痫%磷酰化%STAT3转录因子%滋养层%组织阵列分析
先兆子癇%燐酰化%STAT3轉錄因子%滋養層%組織陣列分析
선조자간%린선화%STAT3전록인자%자양층%조직진렬분석
Pre-eclampsia%Phosphorylation%STAT3 transcription factor%Trophoblasts%Tissue array analysis
目的 构建子痫前期患者绒毛外滋养细胞(extravillous trophoblast,EVCT)组织芯片,并利用该组织芯片检测磷酸化信号转导和转录活化因子3 (phosphorylated signal transducer and activator of transcription 3,pStat3)在子痫前期EVCT中的表达水平,探讨Stat3信号途径在子痫前期病理过程中的作用. 方法 收集2007年12月12日至2010年12月31日郑州大学第三附属医院80例子痫前期和58例正常妊娠孕妇的胎盘组织用来构建组织芯片,利用波形蛋白、细胞角蛋白和人类白细胞抗原-G抗体进行免疫组织化学染色验证EVCT组织芯片,采用免疫组织化学半定量方法检测pStat3在子痫前期和正常妊娠孕妇胎盘EVCT组织的表达情况.采用非参数秩和检验、Kruskai-Wallis H检验、t检验和卡方检验进行统计分析. 结果 57例子痫前期(109个组织点)和31例正常妊娠孕妇的胎盘块(65个组织点)适于EVCT组织芯片的构建.组织芯片中EVCT细胞角蛋白染色阳性,波形蛋白染色阴性,人类白细胞抗原-G染色阳性,符合EVCT的特点,86.4％ (76/88)的样本保留了EVCT,组织芯片构建成功.子痫前期患者胎盘EVCT中pStat3阳性率为51.1％(24/47),重度子痫前期组阳性率为52.3％(23/44),早发型子痫前期组阳性率为50.0％(19/38),均显著低于正常妊娠组的72.4％ (21/29),差异均有统计学意义(U分别为492.00、473.00和401.00,P均＜0.05). 结论 EVCT组织芯片构建成功,利用该芯片可高通量检测pStat3的表达情况.pStat3信号转导途径可能参与了早发型和重度子痫前期的发生发展.
目的 構建子癇前期患者絨毛外滋養細胞(extravillous trophoblast,EVCT)組織芯片,併利用該組織芯片檢測燐痠化信號轉導和轉錄活化因子3 (phosphorylated signal transducer and activator of transcription 3,pStat3)在子癇前期EVCT中的錶達水平,探討Stat3信號途徑在子癇前期病理過程中的作用. 方法 收集2007年12月12日至2010年12月31日鄭州大學第三附屬醫院80例子癇前期和58例正常妊娠孕婦的胎盤組織用來構建組織芯片,利用波形蛋白、細胞角蛋白和人類白細胞抗原-G抗體進行免疫組織化學染色驗證EVCT組織芯片,採用免疫組織化學半定量方法檢測pStat3在子癇前期和正常妊娠孕婦胎盤EVCT組織的錶達情況.採用非參數秩和檢驗、Kruskai-Wallis H檢驗、t檢驗和卡方檢驗進行統計分析. 結果 57例子癇前期(109箇組織點)和31例正常妊娠孕婦的胎盤塊(65箇組織點)適于EVCT組織芯片的構建.組織芯片中EVCT細胞角蛋白染色暘性,波形蛋白染色陰性,人類白細胞抗原-G染色暘性,符閤EVCT的特點,86.4％ (76/88)的樣本保留瞭EVCT,組織芯片構建成功.子癇前期患者胎盤EVCT中pStat3暘性率為51.1％(24/47),重度子癇前期組暘性率為52.3％(23/44),早髮型子癇前期組暘性率為50.0％(19/38),均顯著低于正常妊娠組的72.4％ (21/29),差異均有統計學意義(U分彆為492.00、473.00和401.00,P均＜0.05). 結論 EVCT組織芯片構建成功,利用該芯片可高通量檢測pStat3的錶達情況.pStat3信號轉導途徑可能參與瞭早髮型和重度子癇前期的髮生髮展.
목적 구건자간전기환자융모외자양세포(extravillous trophoblast,EVCT)조직심편,병이용해조직심편검측린산화신호전도화전록활화인자3 (phosphorylated signal transducer and activator of transcription 3,pStat3)재자간전기EVCT중적표체수평,탐토Stat3신호도경재자간전기병리과정중적작용. 방법 수집2007년12월12일지2010년12월31일정주대학제삼부속의원80례자간전기화58례정상임신잉부적태반조직용래구건조직심편,이용파형단백、세포각단백화인류백세포항원-G항체진행면역조직화학염색험증EVCT조직심편,채용면역조직화학반정량방법검측pStat3재자간전기화정상임신잉부태반EVCT조직적표체정황.채용비삼수질화검험、Kruskai-Wallis H검험、t검험화잡방검험진행통계분석. 결과 57례자간전기(109개조직점)화31례정상임신잉부적태반괴(65개조직점)괄우EVCT조직심편적구건.조직심편중EVCT세포각단백염색양성,파형단백염색음성,인류백세포항원-G염색양성,부합EVCT적특점,86.4％ (76/88)적양본보류료EVCT,조직심편구건성공.자간전기환자태반EVCT중pStat3양성솔위51.1％(24/47),중도자간전기조양성솔위52.3％(23/44),조발형자간전기조양성솔위50.0％(19/38),균현저저우정상임신조적72.4％ (21/29),차이균유통계학의의(U분별위492.00、473.00화401.00,P균＜0.05). 결론 EVCT조직심편구건성공,이용해심편가고통량검측pStat3적표체정황.pStat3신호전도도경가능삼여료조발형화중도자간전기적발생발전.
Objective To construct extravillous trophoblast(EVCT) tissue microarray and detect the expression of phosphorylated signal transducer and activator of transcription 3 (pStat3) in EVCT and to explore the role of Stat3 signal transduction pathway in the pathogenesis of preeclampsia.Methods Placentas of 80 pregnant women with preeclampsia and 58 normal pregnant women hospitalized in the Third Affiliated Hospital of Zhengzhou University from December 12,2007 to December 31,2010 were recruited for constructing EVCT tissue microarray.Vimentin,cytokeratin and human leukocyte antigen-G were used to verify EVCT tissue microarray immunohistochemically.The difference of pStat3 expression was detected between preeclampsia patients and normal pregnant women by immunohistochemical staining.Rank sum test,Kruskai-Wallis H test,t-test and Chisquare test were used for statistical analysis.Results Placental tissues from 57 preeclampsia patients (109 tissue cores) and 31 normal pregnant women (65 tissue cores) were suitable for constructing EVCT tissue microarray.The target tissue was positive for both cytokeratin and human leukocyte antigen-G staining and negative for vimentin,which was in accordance with the characters of EVCT tissue.Totally 86.4％(76/88) samples retained the target EVCT tissues,which meant EVCT tissue microarray was constructed successfully.The expression of pStat3 was significantly decreased in EVCT of preeclampsia patients (51.1％,24/47),the early onset (50.0％,19/38) and severe preeclampsia patients(52.3％,23/44) as compared to normal pregnant women (72.4％,21/29) (U=492.00,473.00 and 401.00,P＜0.05 respectively).Conclusions EVCT tissue microarray has been successfully constructed,and could be used to detect pStat3 expression.pStat3 signal transduction pathway may be involved in the development of preeclampsia.
