CAJ | 학술논문

用不同浓度蔗糖、硼酸对云南越桔花粉进行离体培养后统计其萌发率，结果表明：云南越桔花粉最适宜的离体培养基为15％蔗糖＋0．1％硼酸＋1％琼脂，花粉萌发最好，萌发率高达78．26％。此外，4℃恒温、避光条件下贮藏了不同时间的花粉，在适宜培养基中的离体培养萌发率试验表明：云南越桔花粉现采的活力最高，花粉的贮藏时间以不超过1个月为宜。
용불동농도자당、붕산대운남월길화분진행리체배양후통계기맹발솔，결과표명：운남월길화분최괄의적리체배양기위15％자당＋0．1％붕산＋1％경지，화분맹발최호，맹발솔고체78．26％。차외，4℃항온、피광조건하저장료불동시간적화분，재괄의배양기중적리체배양맹발솔시험표명：운남월길화분현채적활력최고，화분적저장시간이불초과1개월위의。
Different concentrations of sucrose and boric acid were used for the isolated culture of pollens in Vaccinium du-clouxii, and the germination rate of the pollens in vitro was statistically analyzed .The results indicated that the optimum culture me-dium for Vaccinium duclouxii pollens in vitro was 15%sucrose +0.1%boric acid +1%agar, and the germination rate of the pol-lens in this culture medium was the highest , reaching 78.26%.In addition, the isolated Vaccinium duclouxii pollens which have been preserved under the conditions of constant temperature 4℃and darkness for a different time were cultivated on suitable culture medium , and their germination rate was tested .The experimental results showed that the fresh -collected pollens had the highest vi-tality, and the storage time for the pollens should not be longer than one month .