CAJ | 학술논문

J-chain是由浆细胞产生的约15KDa大小的糖蛋白，其可以通过与IgM 和IgA尾部半胱氨酸的连接启动多聚化过程并利用二硫键共价稳定地与多聚Ig A和Ig M 结合。本研究克隆并分析了黑眉锦蛇（以下简称蛇）J-chain基因在各组织中的表达情况，发现与其它物种一致，J-chain基因中用于形成二硫键的半胱氨酸残基是高度保守的；蛇J-chain基因在脾脏、肠道中的表达量相对较高，这也正符合免疫球蛋白基因在各组织中的表达情况。蛇J-chain基因的克隆为研究其多聚免疫球蛋白的特点奠定了良好的基础。
J-chain시유장세포산생적약15KDa대소적당단백，기가이통과여IgM 화IgA미부반광안산적련접계동다취화과정병이용이류건공개은정지여다취Ig A화Ig M 결합。본연구극륭병분석료흑미금사（이하간칭사）J-chain기인재각조직중적표체정황，발현여기타물충일치，J-chain기인중용우형성이류건적반광안산잔기시고도보수적；사J-chain기인재비장、장도중적표체량상대교고，저야정부합면역구단백기인재각조직중적표체정황。사J-chain기인적극륭위연구기다취면역구단백적특점전정료량호적기출。
The plasma cell produces the J-chain ,which is a 15-kDa glycoprotein that covalently associates by disulfide linkages with polymeric IgA and IgM .Through the linkage between J -chain and the caudal cysteines of Ig ,the polymerization process could startover .This study cloned the J-chain gene in Elaphe taeniura (hereinafter referred to as snake ) and analyzed the expression levels of it in different tissues .In line with other species ,the cysteine residues used to form disulfide linkages are highly conserved in the snake J-chain gene;Quantitative real-time PCR showed that the J-chain gene was highly expressed in the spleen and intestine ,which is in accordance with other immunoglobulin genes .The cloning of the snake J-chain gene laid a good foundation for researching the polymeric immunoglobulins in snakes .