CAJ | 학술논문

为分子标记辅助选择崂山奶山羊高繁殖力提供依据，对100只崂山奶山羊耳组织采样，利用PCR-SSCP技术对其BM PR-IB基因外显子进行多态性分析。结果表明，崂山奶山羊BM PR -IB基因外显子的820bp位点出现了G→C的突变，A与B等位基因的频率分别为0.855、0.145，此突变位点杂合度为0.248，有效等位基因数为1.330，多态信息含量PIC为0.217，为低度多态。
위분자표기보조선택로산내산양고번식력제공의거，대100지로산내산양이조직채양，이용PCR-SSCP기술대기BM PR-IB기인외현자진행다태성분석。결과표명，로산내산양BM PR -IB기인외현자적820bp위점출현료G→C적돌변，A여B등위기인적빈솔분별위0.855、0.145，차돌변위점잡합도위0.248，유효등위기인수위1.330，다태신식함량PIC위0.217，위저도다태。
One hundred Laoshan milk goats were randomly selected for investigation of polymorphism of BMPR-IB genes by PCR-SSCP .The results suggested the mutation of G→C at the site of 820 bp of the exon of BMPR-IB gene ,and the frequency of allele A and allele B was 0.855 and 0.145 ,respectively ,loci heterozygosity was 0.248 ,the effective number of alleles was 1.330 ,and the polymorphism information content PIC was 0.217 .The findings could provide a reference for MAS programs for high reproduction performance of Laoshan Milk Goat breeds .