CAJ | 학술논문

使用质量浓度为400 m g/L的乙烯利水剂对大豆进行喷施处理，探讨乙烯对大豆花粉育性的调节作用。结果表明，外源乙烯处理后，大豆植株的成花量与花粉数量极显著低于对照，而花粉败育率极显著高于对照，外源乙烯处理对大豆花粉育性的抑制作用十分明显；乙烯利与对照处理基因的差异表达谱及实时定量PC R研究结果表明，细胞应答饥饿、花粉管生长、花粉外壁形成等多个大豆花粉发育相关基因的表达出现显著差异；相同时间段相比，乙烯处理的花芽组织中可溶性糖、可溶性淀粉、超氧化物歧化酶与过氧化物酶活性显著低于对照，而丙二醛含量显著高于对照。本研究为外源乙烯制剂调控大豆育性研究提供了理论依据。
사용질량농도위400 m g/L적을희이수제대대두진행분시처리，탐토을희대대두화분육성적조절작용。결과표명，외원을희처리후，대두식주적성화량여화분수량겁현저저우대조，이화분패육솔겁현저고우대조，외원을희처리대대두화분육성적억제작용십분명현；을희리여대조처리기인적차이표체보급실시정량PC R연구결과표명，세포응답기아、화분관생장、화분외벽형성등다개대두화분발육상관기인적표체출현현저차이；상동시간단상비，을희처리적화아조직중가용성당、가용성정분、초양화물기화매여과양화물매활성현저저우대조，이병이철함량현저고우대조。본연구위외원을희제제조공대두육성연구제공료이론의거。
Heterosis is a very important genetic phenomenon , and its application can significantly improve yields and enhance resistance in many crops . The use of this important tool , however , is limited in soybean production because: 1 ) The small size of soybean flowers makes it difficult and expensive to emasculate and hybridize;2) There are only a limited number of male sterile lines available for hybrid seed production . Therefore , illuminating the mechanisms of male sterile in soybeans will benefit heterosis research of soybean . Previous studies showed that ethylene biosynthesis in the soybean flower was closely related with male sterility . However , the mechanisms that ethylene induced male sterility of soybean remains unknown . Thus ,exogenous ethylene treatment was carried out to study its fertility regulation role in soybean . Scanning electron microscopy (SEM) and palynology methods were used to study the effects of ethylene treatment ,and real time quantitative PCR ( RT-qPCR) was used to evaluate gene expression . The results showed that flower numbers and pollen amount were significantly decreased , and abortion ratio of pollen was significantly promoted in the ethylene treatment compared with the control . The results of difference gene expression profile and RT-qPCR of the control and exogenous ethylene treatments indicated that the expression of eight genes which are related to cellular response to starvation , pollen growth and pollen wall assembly was changed significantly in the ethylene treatment compared with the control . Soluble sugar and starch content , activities of superoxide dismutase and peroxidase were all significantly lower while malondialdehyde content was significantly higher in flower tissue after ethephon treatment than the control . In conclusion , it is confirmed that exogenous ethylene treatment will cause male sterility in soybeans , and the expression of eight genes responsible for pollen development has been significantly changed after ethylene treatment . Carbon starvation and proactive enzyme injury are involved in the process of male sterility formation . The study above provides a theoretical basis for future research on pollen sterility regulated by exogenous ethylene in soybean .