CAJ | 학술논문

为了对新城疫病毒（Newcastle disease virus，NDV）的细胞毒性T细胞（cytotoxic T cell，CTL）表位进行研究，本研究通过在线分析软件预测了NDV弱毒株La Sota和强毒株Herts/33 NP蛋白的MHC I类分子限制性表位，共获得9条可能的表位多肽序列，并人工合成了这些短肽。分别构建了含有La Sota和Herts/33 NP基因的DNA重组质粒并免疫4周龄SPF级C57BL/6小鼠，首免后21 d加强免疫1次，二免后10 d无菌采集脾脏制备脾淋巴细胞悬液，采用ELISpot法测定预测多肽诱导脾淋巴细胞分泌IFN-γ的能力，根据分泌IFN-γ形成的特异性斑点数进行生物统计学分析，发现2条多肽产生的斑点数显著高于无关肽刺激组（P＜0.01）。确定了针对小鼠H-2 kb的限制性T细胞抗原表位的2条多肽。这些可用于合成特异性的MHC-肽四聚体，为后续研究NDV与树突状细胞（dendritic cells，DC）及T淋巴细胞之间的免疫抑制机制提供重要保障。
위료대신성역병독（Newcastle disease virus，NDV）적세포독성T세포（cytotoxic T cell，CTL）표위진행연구，본연구통과재선분석연건예측료NDV약독주La Sota화강독주Herts/33 NP단백적MHC I류분자한제성표위，공획득9조가능적표위다태서렬，병인공합성료저사단태。분별구건료함유La Sota화Herts/33 NP기인적DNA중조질립병면역4주령SPF급C57BL/6소서，수면후21 d가강면역1차，이면후10 d무균채집비장제비비림파세포현액，채용ELISpot법측정예측다태유도비림파세포분비IFN-γ적능력，근거분비IFN-γ형성적특이성반점수진행생물통계학분석，발현2조다태산생적반점수현저고우무관태자격조（P＜0.01）。학정료침대소서H-2 kb적한제성T세포항원표위적2조다태。저사가용우합성특이성적MHC-태사취체，위후속연구NDV여수돌상세포（dendritic cells，DC）급T림파세포지간적면역억제궤제제공중요보장。
The NP protein MHC I restricted epitopes of attenuated strain La Sota and virulent strain Herts/33 of Newcastle disease virus (NDV) were predicted using online analysis software for localization of cytotoxic T cell (CTL) epitopes. A total of 9 possible epitope sequences were identified and synthesized. The DNA plasmids were constructed to contain La Sota, Herts/33 strain NP genes and then used to immunize 4-week-old SPF C57BL/6 mice. A booster immunization was performed at day 21 post the first vaccination. Spleens of immunized mice were aseptically collected at day 10 post the second immunization and lymphocyte suspensions were prepared to detect IFN-γsecretions in ELISpot. Specific spots of lymphocyte IFN-γsecretions were analyzed. Two out of 9 peptides showed significantly higher levels of lymphocyte IFN-γsecretions than irrelevance peptide (p<0.01). As a result, two restricted T cell epitopes were determined on mouse H-2 kb. Information can be used for synthesis of specific MHC-peptide tetramer for further investigation into the mechanisms of immunosuppression between NDV and dendritic cells (DC) and T lymphocytes.