CAJ | 학술논문

溶杆菌属细菌在植物病害生物防治中有着广阔的应用潜力。本文以本实验室从中药材远志分离筛选的溶杆菌属新菌株Lysobacter sp. SNNU513为材料，筛选出高效制备该菌株感受态细胞Inoue法，电击转化条件为场强20 kV/cm、电脉冲时间5 ms时可将含绿色荧光蛋白基因gfp的质粒pGLO导入该菌株感受态细胞中，重组菌SNNU513-pGLO能高效、稳定表达绿色荧光蛋白基因，与出发菌株的生长特性、抑菌活性等生物学特性差异不显著。将成功构建的重组菌SNNU513-pGLO用于检测该菌株在玉米根部的定殖规律，结果表明，定殖量从表皮到韧皮部有明显减少趋势。
용간균속세균재식물병해생물방치중유착엄활적응용잠력。본문이본실험실종중약재원지분리사선적용간균속신균주Lysobacter sp. SNNU513위재료，사선출고효제비해균주감수태세포Inoue법，전격전화조건위장강20 kV/cm、전맥충시간5 ms시가장함록색형광단백기인gfp적질립pGLO도입해균주감수태세포중，중조균SNNU513-pGLO능고효、은정표체록색형광단백기인，여출발균주적생장특성、억균활성등생물학특성차이불현저。장성공구건적중조균SNNU513-pGLO용우검측해균주재옥미근부적정식규률，결과표명，정식량종표피도인피부유명현감소추세。
Lysobacter sp. SNNU513 isolated from Radix polygalae rhizospher could inhibit pathogenic fungus. In this study, the preparation of the efficient competent cells were screened out and pGLO plasmid was introduced into the competent cells by electroporation and transformation. The results showed that the recombinant strain SNNU513-pGLO could efficiently and stably express the green fluorescent protein gene (gfp) when the electric field strength was 20 kV/cm and the electric pulse time was 5 ms. The percent of the green cells was 100%when the strain SNNU513-pGLO was recirculated in the non-selective medium for 20 times. Both strains SNNU513-pGLO and SNNU513 showed the same growth characteristics and the same inhibition of Rhizotonia cerealis in vivo. The gene gfp was transferred into original strain Lysobacter sp. SNNU513 by electroporation successfully. The gfp-tagged strain adhering more tightly to the velamen than the phloem could colonize on the surface and interior of maize’s root.