CAJ | 학술논문

本研究前期成功分离获得1株对水稻真菌病害具有较强抑制作用的枯草芽孢杆菌T429，为开发生防菌农药新剂型，利用喷雾干燥法对生防菌 T429发酵液进行处理，以喷雾干燥后产物活菌量为主要指标，采用单因素和正交试验法，对影响喷雾干燥产物指标的主要因素进行优化。结果表明，最佳喷雾干燥条件为进风温度120℃、进样速度720 mL/h、雾化口径1 mm、雾化压力0.1 MPa、热风流量30 M3/h，可获得产物活菌量达8.61×109 cfu/g。该工艺稳定、可控，为以枯草芽孢杆菌为核心的微生物杀菌剂新剂型的研发提供了科学依据。
본연구전기성공분리획득1주대수도진균병해구유교강억제작용적고초아포간균T429，위개발생방균농약신제형，이용분무간조법대생방균 T429발효액진행처리，이분무간조후산물활균량위주요지표，채용단인소화정교시험법，대영향분무간조산물지표적주요인소진행우화。결과표명，최가분무간조조건위진풍온도120℃、진양속도720 mL/h、무화구경1 mm、무화압력0.1 MPa、열풍류량30 M3/h，가획득산물활균량체8.61×109 cfu/g。해공예은정、가공，위이고초아포간균위핵심적미생물살균제신제형적연발제공료과학의거。
Bacillus subtilis T429 was confirmed to be highly bacteriostatic to rice fungus diseases in previous study. Fermentation broth of B. subtilis T429 was granulated by spray-drying technology to develop new bacterial pesticide formulation. Single factor test and experiment with four-factor and three-level orthogonal were carried out to optimize spray-drying condition. Results showed that the optimum conditions were inlet air temperature 120℃, the feed flow rate 720 mL/h, atomization caliber 1 mm, atomization pressure 0.1 MPa, hot air flow 30 M3/h. The number of viable cells of products reached to 8.61×109 cfu/g under the optimal conditions. These data helps development of new pesticide formulation of B. subtilis.