CAJ | 학술논문

哈茨木霉SH2303是本实验室分离获得的1株具有生防效果的菌株，该菌株能够较好的防治玉米小斑病。通过离体叶片试验确定哈茨木霉SH2303诱导玉米抗小斑病的持效期达15 d。盆栽及大田试验表明，防治玉米小斑病防效分别达到78.1%和56.3%。盆栽试验表明，哈茨木霉 SH2303处理的叶片在挑战接种小斑病菌后第36 h，玉米体内防御反应酶系PAL和SOD活性达到峰值。同时，防御基因Pal和Sod的表达水平也明显上升。综合分析表明，哈茨木霉SH2303的诱导抗性作用是防治玉米小斑病的重要机制之一。
합자목매SH2303시본실험실분리획득적1주구유생방효과적균주，해균주능구교호적방치옥미소반병。통과리체협편시험학정합자목매SH2303유도옥미항소반병적지효기체15 d。분재급대전시험표명，방치옥미소반병방효분별체도78.1%화56.3%。분재시험표명，합자목매 SH2303처리적협편재도전접충소반병균후제36 h，옥미체내방어반응매계PAL화SOD활성체도봉치。동시，방어기인Pal화Sod적표체수평야명현상승。종합분석표명，합자목매SH2303적유도항성작용시방치옥미소반병적중요궤제지일。
Trichoderma harzianum SH2303 with high biocontrol activity against southern corn leaf blight (SCLB) was isolated from field soil. Pretreated detached maize leaves with T. harzianum SH2303 showed induced resistance to SCLB with a duration of 15 days. Efficacy of T. harzianum SH2303 against SCLB reached 78.1%and 56.3%under greenhouse and field conditions respectively when spraying spore suspension over maize seedling leaves prior to inoculation of the pathogen. The activities of phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) in maize leaves peaked at 36 h in the Trichoderma treatments after pathogen challenging inoculation. RNA expression levels of Pal and Sod were also upregulated in pace with the enzyme activity. Results indicated that induced resistance might be one of the important mechanisms in the biocontrol of SCLB by T. harzianum SH2303.