CAJ | 학술논문

本文以单糖葡萄糖、果糖、蔗糖、葡萄糖＋果糖（1：1）、多糖可溶性淀粉以及可溶性淀粉经酶处理后的水解产物为碳源，测定了发酵过程中糖消耗水平及绿僵菌生物量，分析了碳源对绿僵菌生长代谢的影响。结果表明，供试碳源中，葡萄糖＋果糖的生物量最大（16.16 g/L）、达到最大生物量时的增长速度也最大（0.4238 g/L·h），并且被绿僵菌利用最快（48 h）；其它依次为蔗糖、葡萄糖、果糖、可溶性淀粉；相同质量淀粉经外源α-淀粉酶水解12 h后作为碳源，与直接利用可溶性淀粉相比绿僵菌生物量增加14.3%左右。由此可见多糖基质碳源不利于绿僵菌的吸收利用，若在多糖基质碳源中添加外源糖类水解酶提前将多糖降解为单糖供绿僵菌生长，可提高绿僵菌对其利用率，进而提高发酵水平，降低发酵成本。
본문이단당포도당、과당、자당、포도당＋과당（1：1）、다당가용성정분이급가용성정분경매처리후적수해산물위탄원，측정료발효과정중당소모수평급록강균생물량，분석료탄원대록강균생장대사적영향。결과표명，공시탄원중，포도당＋과당적생물량최대（16.16 g/L）、체도최대생물량시적증장속도야최대（0.4238 g/L·h），병차피록강균이용최쾌（48 h）；기타의차위자당、포도당、과당、가용성정분；상동질량정분경외원α-정분매수해12 h후작위탄원，여직접이용가용성정분상비록강균생물량증가14.3%좌우。유차가견다당기질탄원불리우록강균적흡수이용，약재다당기질탄원중첨가외원당류수해매제전장다당강해위단당공록강균생장，가제고록강균대기이용솔，진이제고발효수평，강저발효성본。
The effect of different carbon sources on the fermentation of Metarhizium anisopliae IMI330189 was determined in this paper. The effects of six carbon sources-glucose, sucrose, soluble starch, fructose, glucose+fluctose (1:1) and the starch hydrolysate on the growth and the metabolism of M. anisopliae were discussed. The results showed that glucose+fluctose was the best carbon source among the six carbon sources, the biomass, growth rate and degradation time were 16.16 g/L, 0.4238 g/L·h, 48 h respectively, and the biomass were glucose+fluctose>sucrose>glucose>fructose>soluble starch. The starch hydrolysate were found to be better for M. anisopliae, the biomass of M. anisopliae was improved 14.3% compared with soluble starch. So it was demonstrated that the utilization of polysaccharide was insufficient for M. anisopliae and the addition of exogenous enzyme might improve the fermentation level and decrease the cost of M. anisopliae.