CAJ | 학술논문

在27～29℃温度下，用1.5 mL/L的正己烷蒸气处理菜蛾盘绒茧蜂滞育蛹10～15 min后可以解除其滞育。为探讨解除滞育的机制，测定了正己烷处理后菜蛾盘绒茧蜂滞育预蛹体内的过氧化氢含量（H2O2）和3种抗氧化酶（SOD、POD和CAT）活性。结果表明正己烷处理后，滞育预蛹体内H2O2含量、SOD和POD活性均迅速上升，CAT活性则下降。就5个取样点的平均值而言，H2O2含量和SOD活性显著高于非滞育预蛹和未经处理的滞育预蛹，POD活性低于非滞育预蛹而高于未经处理的滞育预蛹，CAT活性则低于未经处理的滞育预蛹而高于非滞育预蛹。3种抗氧化酶活性的变化与体内H2O2含量的平衡有关，而H2O2含量的变化可能与体内激素水平的调节有关。
재27～29℃온도하，용1.5 mL/L적정기완증기처리채아반융충봉체육용10～15 min후가이해제기체육。위탐토해제체육적궤제，측정료정기완처리후채아반융충봉체육예용체내적과양화경함량（H2O2）화3충항양화매（SOD、POD화CAT）활성。결과표명정기완처리후，체육예용체내H2O2함량、SOD화POD활성균신속상승，CAT활성칙하강。취5개취양점적평균치이언，H2O2함량화SOD활성현저고우비체육예용화미경처리적체육예용，POD활성저우비체육예용이고우미경처리적체육예용，CAT활성칙저우미경처리적체육예용이고우비체육예용。3충항양화매활성적변화여체내H2O2함량적평형유관，이H2O2함량적변화가능여체내격소수평적조절유관。
Exposure of the diapause individuals to 27-29 ℃ and 1.5 mL/L n-hexane for 10-15 min can effectively terminate diapause in Cotesia vestalis (Haliday). To unravel the mechanism of diapause termination, hydrogen peroxide content and activities of three antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), were quantified in diapause prepupae of C. vestalis. Results showed that, in the n-hexane-exposed diapause individuals, hydrogen peroxide content and activities of SOD and POD increased sharply, while activity of CAT declined. On average, hydrogen peroxide content and SOD activity were significantly higher in the exposed diapause prepupae than those in unexposed both diapause and non-diapause prepupae;POD activity was significantly lower than that in non-diapause prepupae but significantly higher than that in unexposed diapause prepupae; CAT activity was significantly lower than that in the unexposed diapause prepupae but significantly higher than that in non-diapause prepupae. Hydrogen peroxide content depends on activities of the three antioxidant enzymes, while the former can regulate hormone level in diapause prepupae, which may be the reasons for diapause termination by n-hexane.