CAJ | 학술논문

在(25±0.5)℃下,设置4个水流速度实验组,分别为静水对照组0 bl/s(体长/秒)、1.0 bl/s组、2.0 bl/s组和4.0 bl/s组,鲃研究不同水流速度对中华倒刺(Spinibarbus sinensis)幼鱼肌肉自由基代谢的影响,训练周期为8周。结果显示,肌肉超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)的活性随着水流速度的增加而升高,训练组过氧化氢酶(CAT)活性出现不同程度的下降,而抗氧化物谷胱甘肽(GSH)的含量未发生明显变化(P>0.05)；训练组肌肉总抗氧化能力(T-AOC)均高于对照组,2.0 bl/s组最高,4.0 bl/s组次之,1.0 bl/s组最低,且2.0 bl/s和4.0 bl/s组T-AOC与对照组有显著性差异(P<0.05)。8周训练后,2.0 bl/s组丙二醛(MDA)含量显著低于其他3组(P<0.05),而H2O2含量随着水流速度增加呈上升趋势。8周运动训练对骨骼肌B细胞淋巴瘤因子-2(Bcl-2)、Bcl-2相关X蛋白(Bax)的含量及血清肌酸激酶(CK)活性无显著影响(P>0.05),但2.0 bl/s组Bax和Bax/Bcl-2显著低于4.0 bl/s组(P<0.05)。结果表明,2.0 bl/s的水流速鲃度可提高中华倒刺肌肉的抗氧化能力,降低脂质过氧化水平,并在一定程度上抑制肌细胞凋亡。
재(25±0.5)℃하,설치4개수류속도실험조,분별위정수대조조0 bl/s(체장/초)、1.0 bl/s조、2.0 bl/s조화4.0 bl/s조,파연구불동수류속도대중화도자(Spinibarbus sinensis)유어기육자유기대사적영향,훈련주기위8주。결과현시,기육초양화물기화매(SOD)화곡광감태과양화물매(GSH-PX)적활성수착수류속도적증가이승고,훈련조과양화경매(CAT)활성출현불동정도적하강,이항양화물곡광감태(GSH)적함량미발생명현변화(P>0.05)；훈련조기육총항양화능력(T-AOC)균고우대조조,2.0 bl/s조최고,4.0 bl/s조차지,1.0 bl/s조최저,차2.0 bl/s화4.0 bl/s조T-AOC여대조조유현저성차이(P<0.05)。8주훈련후,2.0 bl/s조병이철(MDA)함량현저저우기타3조(P<0.05),이H2O2함량수착수류속도증가정상승추세。8주운동훈련대골격기B세포림파류인자-2(Bcl-2)、Bcl-2상관X단백(Bax)적함량급혈청기산격매(CK)활성무현저영향(P>0.05),단2.0 bl/s조Bax화Bax/Bcl-2현저저우4.0 bl/s조(P<0.05)。결과표명,2.0 bl/s적수류속파도가제고중화도자기육적항양화능력,강저지질과양화수평,병재일정정도상억제기세포조망。
To explore the effects of exercise training under different velocities on the free radical metabolism of juvenile Spinibarbus sinensis, the experimental fish were divided into four groups at (25±0.5)℃. After the water current treat-ment (0 bl/s, 1.0 bl/s, 2.0 bl/s, 4.0 bl/s) for 8 weeks, the exercise training induced adaption of the antioxidant enzyme system of muscle, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) increased with the increasing water velocity, while the activity of catalase (CAT) dropped in varying degrees. No significant differences in the level of glutathione(GSH) was observed among the treatments with various velocities. Compared with the control group, the content of the total antioxidant capacity (T-AOC) increased in the trained group, the maximum values were in the water velocity of 2.0 bl/s, the second maximum values were in 4.0 bl/s, with significant differences with control (P<0.05). At the end of experiment, the level of MDA in the group of 2.0 bl/s decreased significantly(P<0.05), however the content of H2O2 increased with the increasing water velocity. Compared with the control group, no significant dif-ferences in the content of Bcl-2, Bax and the activity of CK were found among treatments(P>0.05), however the ratio between Bax and Bcl-2 of 4.0 bl/s was significantly higher than that of 2.0 bl/s(P<0.05). The results showed that the exercise training under 2.0 bl/s could effectively improve the antioxidant function, decrease lipid peroxidation level, and inhibit muscle cell apoptosis of juvenile S. sinensis at some level.