CAJ | 학술논문

为评价牛γ-干扰素ELISA检测方法检测牛结核的效果及国产试剂盒的检测效果，本试验首先将国产试剂盒与Prionics试剂盒对42份相对阳性的样品和105份相对阴性的样品进行对比研究。然后对5个规模化牛场的3000头奶牛首先进行国产单纯结核菌素颈部皮内变态反应试验，3天后选取皮内变态反应阳性和可疑及部分阴性牛共418头，进行牛γ-干扰素试验。结果国产试剂盒对阳性和阴性样品的检测敏感性和特异性分别为95.2%和100%，与Priobics试剂盒的符合率为99.3%。表明国产试剂盒与进口试剂盒的检测能力一致，牛γ-干扰素检测方法准确可靠。5个牛场的3000头奶牛单纯结核菌素颈部皮内变态反应试验阳性为138头，可疑105头。γ-干扰素试验对418头奶牛的检测，其中阳性74头，与颈部皮内变态反应（可疑牛暂时视为阴性）的符合率为60.5%。
위평개우γ-간우소ELISA검측방법검측우결핵적효과급국산시제합적검측효과，본시험수선장국산시제합여Prionics시제합대42빈상대양성적양품화105빈상대음성적양품진행대비연구。연후대5개규모화우장적3000두내우수선진행국산단순결핵균소경부피내변태반응시험，3천후선취피내변태반응양성화가의급부분음성우공418두，진행우γ-간우소시험。결과국산시제합대양성화음성양품적검측민감성화특이성분별위95.2%화100%，여Priobics시제합적부합솔위99.3%。표명국산시제합여진구시제합적검측능력일치，우γ-간우소검측방법준학가고。5개우장적3000두내우단순결핵균소경부피내변태반응시험양성위138두，가의105두。γ-간우소시험대418두내우적검측，기중양성74두，여경부피내변태반응（가의우잠시시위음성）적부합솔위60.5%。
In order to evaluate the effectiveness of gamma interferon test to detect bovine tuberculosis and the detection effect of a domestic kit, a comparative study was done between Prionics kits and the domestic kits using 42 positive samples and 105 negative samples. Then 3000 cattle from five intensive farms were first tested with single skin test, and 3 days later, 418 cattle including skin test positive and suspicious ones and some negative ones were tested with gamma interferon test. Results showed that the sensitivity and specificity of the domestic kit were 95.2%and 100%respectively. and the coincidence was 99.3%with the Prionics kit,suggesting that the gamma interferon test was accurate and reli-able for detection of bovine tuberculosis, and the domestic kit was as good as the Prionics kit. There were 138 skin test positive cattle and 105 suspicious cattle from 3000 tested cattle. In gamma interferon test, 74/418 cattle were detected positive, and the coincidence rate was 60.5%with skin test (suspicious cattle considered as negative).