中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2014年
1期
64-66
,共3页
牛结核%γ-干扰素试验%单纯颈部皮试变态反应%比较皮试变态反应
牛結覈%γ-榦擾素試驗%單純頸部皮試變態反應%比較皮試變態反應
우결핵%γ-간우소시험%단순경부피시변태반응%비교피시변태반응
bovine tuberculosis%γ-interferon release assay%simple cervical allergy skin test%comparative aller-gy skin test
目的:评价γ-干扰素释放试验(IGRA)牛结核病检疫过程中的效果。方法在牛结核病检疫过程中对皮试初筛为结核病和疑似结核病的89(74/15)头奶牛同时进行r干扰素试验、单纯颈部皮试变态反应(SCT)和比较皮试变态反应(CCT)检测,结果单纯颈部皮试变态反应二次检疫中89头牛均判为阳性,单纯颈部皮试变态反应与比较皮试变态反应的阳性符合数为67头。阳性符合率为75.3%;γ-干扰素释放试验和单纯颈部皮试变态反应检测两者的阳性符合数为64头,阳性符合率为71.9%;γ-干扰素释放试验与比较皮试变态反应的阳性符合数为58头,阳性符合率为79.5%。阴性符合数为16头,阴性符合率为48.4%;结论γ-干扰素释放试验在保持较高灵敏度的同时,具有比变态反应更好的特异性。
目的:評價γ-榦擾素釋放試驗(IGRA)牛結覈病檢疫過程中的效果。方法在牛結覈病檢疫過程中對皮試初篩為結覈病和疑似結覈病的89(74/15)頭奶牛同時進行r榦擾素試驗、單純頸部皮試變態反應(SCT)和比較皮試變態反應(CCT)檢測,結果單純頸部皮試變態反應二次檢疫中89頭牛均判為暘性,單純頸部皮試變態反應與比較皮試變態反應的暘性符閤數為67頭。暘性符閤率為75.3%;γ-榦擾素釋放試驗和單純頸部皮試變態反應檢測兩者的暘性符閤數為64頭,暘性符閤率為71.9%;γ-榦擾素釋放試驗與比較皮試變態反應的暘性符閤數為58頭,暘性符閤率為79.5%。陰性符閤數為16頭,陰性符閤率為48.4%;結論γ-榦擾素釋放試驗在保持較高靈敏度的同時,具有比變態反應更好的特異性。
목적:평개γ-간우소석방시험(IGRA)우결핵병검역과정중적효과。방법재우결핵병검역과정중대피시초사위결핵병화의사결핵병적89(74/15)두내우동시진행r간우소시험、단순경부피시변태반응(SCT)화비교피시변태반응(CCT)검측,결과단순경부피시변태반응이차검역중89두우균판위양성,단순경부피시변태반응여비교피시변태반응적양성부합수위67두。양성부합솔위75.3%;γ-간우소석방시험화단순경부피시변태반응검측량자적양성부합수위64두,양성부합솔위71.9%;γ-간우소석방시험여비교피시변태반응적양성부합수위58두,양성부합솔위79.5%。음성부합수위16두,음성부합솔위48.4%;결론γ-간우소석방시험재보지교고령민도적동시,구유비변태반응경호적특이성。
Objective To evaluate the effect ofγ-interferon release assay (IGRA) in bovine tuberculosis detection. Method 89(74 positive/15 suspicious) dairy cows preliminarily screened with skin test were tested byγ-inerfer-on release assay (IGRA), simple cervical allergy skin test (SCT) and comparative allergy cervical test (CCT). Result 89 cows were considered as bovine tuberculosis positive in SCT, and 67 of them were considered as posi-tive in CCT with the positive coincidence of 75.3%. 64 cows were considered as positive both in IGRA and SCT with the positive coincidence of 71.9%. 58 cows were considered as positive both in IGRA and CCT, with the positive coin-cidence of 79.5%. 16 cows were considered as negative,with the negative coincidence of 48.4%. Conclusion Theγ-interferon release was of higher sensitivity and better specificity than the other two allergy tests.