CAJ | 학술논문

针对聚合物驱后油藏内源微生物的生长特点，对大庆油田萨南开发区南二区东部聚合物驱后典型油藏开展了激活内源微生物驱油现场试验。通过优选由玉米浆干粉、硝酸钠和磷酸氢二铵构成激活剂，与保护剂聚合物交替注入，并运用分子生态学末端限制性片段长度多态性方法分析了内源微生物激活前后群落结构的变化规律，研究了利用内源微生物驱油技术的可行性。现场试验结果表明：聚合物驱后油藏中有明显的产气增压效果；油藏驱动压力的动态变化及代谢产物气体组分CH4和CO2的δ13 C同位素含量波动，验证了激活剂的加入促使油藏内源微生物微氧和无氧代谢交替进行；产出液的各项生化监测指标均有明显变化，激活后优势菌群为Pseudomonas、Thauera和Arcobacter，且丰度增高；试验区阶段累计增油3068.13 t，含水率下降2.2％，驱油增产效果明显。该试验的成功实施证明了聚合物驱后油藏采用激活内源微生物驱油的工艺方法是可行的，并为同类油藏进一步提高采收率提供了可借鉴的方法和途径。
침대취합물구후유장내원미생물적생장특점，대대경유전살남개발구남이구동부취합물구후전형유장개전료격활내원미생물구유현장시험。통과우선유옥미장간분、초산납화린산경이안구성격활제，여보호제취합물교체주입，병운용분자생태학말단한제성편단장도다태성방법분석료내원미생물격활전후군락결구적변화규률，연구료이용내원미생물구유기술적가행성。현장시험결과표명：취합물구후유장중유명현적산기증압효과；유장구동압력적동태변화급대사산물기체조분CH4화CO2적δ13 C동위소함량파동，험증료격활제적가입촉사유장내원미생물미양화무양대사교체진행；산출액적각항생화감측지표균유명현변화，격활후우세균군위Pseudomonas、Thauera화Arcobacter，차봉도증고；시험구계단루계증유3068.13 t，함수솔하강2.2％，구유증산효과명현。해시험적성공실시증명료취합물구후유장채용격활내원미생물구유적공예방법시가행적，병위동류유장진일보제고채수솔제공료가차감적방법화도경。
Based on the characteristics of indigenous microbial growth in reservoirs after polymer flooding (RAPFs),a field test wasconducted to activate indigenous microorganisms for microbial enhanced oil recovery (MEOR)in representative RAPFs in the east ofNan-2 district in Sanan development zone,Daqing oilfield.The optimized activator,which consisted of corn steep powder,NaNO3,and (NH4)2 HPO3,was injected alternately with protectant polymer.The changes in indigenous microbial community structure be-fore and after activation were examined using terminal restriction fragment length polymorphism analysis,in order to determine thefeasibility for application of indigenous MEOR technique.Results showed that:there existed a significant gas supercharging effect inthe RAPFs;Dynamic changes in the driving pressure of RAPFs and substantial fluctuations in theδ13 C isotope contents of microbial-ly produced CH4 and CO2 confirmed that the addition of activator promoted alternative micro-aerobic and anaerobic metabolism of in-digenous microorganisms in the reservoir;there were significant changes in the major biochemical parameters of the produced fluidafter activation of indigenous microorganisms;the dominant indigenous microbial populations included Pseudomonas,Thauera,andArcobacter,whose abundance experienced great increases after activation;the incremental oil production was 3 068.13 t while thewater content declined by 2.2%,indicating that MEOR application significantly improved the oil recovery.This work proved that itis feasible to activate indigenous microorganisms for MEOR in RAPFs,which provides an alternative approach for further improvingoil recovery in similar reservoirs.