CAJ | 학술논문

目的：分析不同肝病患者血清microRNA-21（miR-21）水平，以探讨miR-21对肝细胞癌（HCC）的诊断价值。方法以实时定量逆转录PCR法检测正常人、慢性乙型肝炎（CHB）、肝硬化和HCC患者（各组均为25例）血清miR-21水平，分析miR-21水平与HCC临床病理学特征的关系。结果正常人、CHB和肝硬化患者血清miR-21相对水平分别为（1.1±1.7）、（2.3±2.6）和（2.8±2.5），而HCC患者为（22.6±4.4），显著高于前三组（P<0.001）；HCC患者术后1w和1m血清miR-21相对水平分别为（18.4±3.5）和（3.1±2.7），均较术前显著降低（P＜0.001）；HCC患者血清miR-21水平与肿瘤大小、癌栓以及HBV感染相关，与肿瘤分化程度、数目和血清AFP水平无相关性。结论 miR-21在HCC患者血清中显著升高，可能作为HCC早期诊断的潜在标志。
목적：분석불동간병환자혈청microRNA-21（miR-21）수평，이탐토miR-21대간세포암（HCC）적진단개치。방법이실시정량역전록PCR법검측정상인、만성을형간염（CHB）、간경화화HCC환자（각조균위25례）혈청miR-21수평，분석miR-21수평여HCC림상병이학특정적관계。결과정상인、CHB화간경화환자혈청miR-21상대수평분별위（1.1±1.7）、（2.3±2.6）화（2.8±2.5），이HCC환자위（22.6±4.4），현저고우전삼조（P<0.001）；HCC환자술후1w화1m혈청miR-21상대수평분별위（18.4±3.5）화（3.1±2.7），균교술전현저강저（P＜0.001）；HCC환자혈청miR-21수평여종류대소、암전이급HBV감염상관，여종류분화정도、수목화혈청AFP수평무상관성。결론 miR-21재HCC환자혈청중현저승고，가능작위HCC조기진단적잠재표지。
Objective To evaluate serum level of microRNA-21 (miR-21) in normal persons and patients with different liver diseases,and to investigate its effect in the diagnosis of hepatocellular carcinoma(HCC). Methods The serum level of miR-21 in healthy controls (n=25),patients with chronic hepatitis B(CHB,n=25), cirrhosis (n=25),and HCC (n=25) were measured by real-time quantitative RT-PCR. The relationships between serum miR-21 levels and clinical pathological features of HCC were also investigated. Results The relative serum levels of miR-21 in normal controls,CHB and cirrhotic patients were (1.1±1.7),(2.3±2.6) and (2.8±2.5),respec-tively,all of which were significantly lower than that in HCC patients(22.6±4.4),P<0.001;The relative serum levels of miR-21 in HCC patients one week and one month after surgery were(18.4±3.5) and (3.1±2.7),respectively,sig-nificantly lower than that before operation (22.6±4.4),P<0.001;Serum levels of miR-21 were correlated with tumor size,tumor thrombosis and HBV infection,but not with tumor numbers,tumor differentiation and serum AFP levels. Conclusions Serum levels of miR-21 is elevated in HCC patients and might be used as a potential biomarker for the early diagnosis of HCC.