实用肝脏病杂志
實用肝髒病雜誌
실용간장병잡지
JOURNAL OF CLINICAL HEPATOLOGY
2014年
1期
51-54
,共4页
代雪枫%马勇%徐长江%王莉
代雪楓%馬勇%徐長江%王莉
대설풍%마용%서장강%왕리
慢性HBV携带者%凋亡%Fas%Fas配体
慢性HBV攜帶者%凋亡%Fas%Fas配體
만성HBV휴대자%조망%Fas%Fas배체
Chronic asymptomatic hepatitis B virus carrier%Apoptosis%Fas%Fas ligand
目的：观察慢性HBV携带者（chronic asymptomatic HBV carrier，ASC）肝组织Fas和Fas配体（FasL）的表达和血清可溶性Fas（sFas）水平的变化。方法对120例ASC行肝穿刺，采用免疫组织化学法检测肝组织Fas和FasL表达，采用ELISA法检测血清sFas水平。结果120例ASC肝组织完全正常者仅占11%，轻度肝炎占59%，而中、重度肝炎占30%；表现正常的肝组织无Fas和FasL表达，轻度肝炎肝组织多无表达或仅限于界面炎症区的肝细胞及淋巴细胞阳性表达，而中、重度肝炎肝组织Fas和FasL多为阳性，Fas强阳性率分别达34.8%和69.2%，FasL强阳性率分别达30.4%和53.8%，均明显高于正常组和轻度炎症组（P<0.05），同时中、重度肝炎组间也存在显著性差异（P<0.05），其分布除界面炎症区外，肝小叶中也弥漫分布；肝组织表现正常的ASC血清sFas水平为（1.68±0.33） ng/L，而轻、中、重度肝炎患者则分别为（2.58±0.31）ng/L、（3.94±0.21）ng/L和（5.94±0.26）ng/L，均较正常组显著升高（F=218.01，P<0.01），各组间sFas水平也均具有统计学差异（P<0.01）。结论 Fas和FasL介导的肝细胞凋亡在慢性HBV携带者肝组织病变中可能起到了重要作用。
目的：觀察慢性HBV攜帶者（chronic asymptomatic HBV carrier，ASC）肝組織Fas和Fas配體（FasL）的錶達和血清可溶性Fas（sFas）水平的變化。方法對120例ASC行肝穿刺，採用免疫組織化學法檢測肝組織Fas和FasL錶達，採用ELISA法檢測血清sFas水平。結果120例ASC肝組織完全正常者僅佔11%，輕度肝炎佔59%，而中、重度肝炎佔30%；錶現正常的肝組織無Fas和FasL錶達，輕度肝炎肝組織多無錶達或僅限于界麵炎癥區的肝細胞及淋巴細胞暘性錶達，而中、重度肝炎肝組織Fas和FasL多為暘性，Fas彊暘性率分彆達34.8%和69.2%，FasL彊暘性率分彆達30.4%和53.8%，均明顯高于正常組和輕度炎癥組（P<0.05），同時中、重度肝炎組間也存在顯著性差異（P<0.05），其分佈除界麵炎癥區外，肝小葉中也瀰漫分佈；肝組織錶現正常的ASC血清sFas水平為（1.68±0.33） ng/L，而輕、中、重度肝炎患者則分彆為（2.58±0.31）ng/L、（3.94±0.21）ng/L和（5.94±0.26）ng/L，均較正常組顯著升高（F=218.01，P<0.01），各組間sFas水平也均具有統計學差異（P<0.01）。結論 Fas和FasL介導的肝細胞凋亡在慢性HBV攜帶者肝組織病變中可能起到瞭重要作用。
목적：관찰만성HBV휴대자（chronic asymptomatic HBV carrier，ASC）간조직Fas화Fas배체（FasL）적표체화혈청가용성Fas（sFas）수평적변화。방법대120례ASC행간천자，채용면역조직화학법검측간조직Fas화FasL표체，채용ELISA법검측혈청sFas수평。결과120례ASC간조직완전정상자부점11%，경도간염점59%，이중、중도간염점30%；표현정상적간조직무Fas화FasL표체，경도간염간조직다무표체혹부한우계면염증구적간세포급림파세포양성표체，이중、중도간염간조직Fas화FasL다위양성，Fas강양성솔분별체34.8%화69.2%，FasL강양성솔분별체30.4%화53.8%，균명현고우정상조화경도염증조（P<0.05），동시중、중도간염조간야존재현저성차이（P<0.05），기분포제계면염증구외，간소협중야미만분포；간조직표현정상적ASC혈청sFas수평위（1.68±0.33） ng/L，이경、중、중도간염환자칙분별위（2.58±0.31）ng/L、（3.94±0.21）ng/L화（5.94±0.26）ng/L，균교정상조현저승고（F=218.01，P<0.01），각조간sFas수평야균구유통계학차이（P<0.01）。결론 Fas화FasL개도적간세포조망재만성HBV휴대자간조직병변중가능기도료중요작용。
Objective To investigate the expression of Fas antigen and Fas ligand (FasL) in liver tissues from chronic asymptomatic hepatitis B virus (HBV) carrier (ASC). Methods Liver biopsy specimen and sera from 120 individuals with ASC were obtained;The expression of Fas and FasL in liver tissues were detected by immunohistostaining. Serum levels of soluble Fas (sFas) and sFasL were measured by ELISA. Results Only 11%had a normal appearance of liver tissues in the 120 chronic HBV carriers,and 59% had mild and other 30% had moderate to severe hepatitis;Fas and FasL were negative in normal liver tissues,almost absent or only slightly pos-itive in hepatocytes and lymphocytes in inflammatory zone of the interface in liver tissues with mild hepatitis ,and strongly positive in those with moderate or severe hepatitis,showing a pattern of diffused staining in the interface area of hepatic lobules;The strongly positive rate for Fas and FasL in moderate (34.8% and 30.4%,respectively) and severe hepatitis (69.2% and 53.8%,respectively) was significantly higher than that in mild hepatitis or normal liver tissues(P<0.05);Serum sFas from persons with normal liver tissues was(1.68±0.33) ng/L,and they increased significantly to (2.58±0.31) ng/L,(3.94±0.21) ng/L and (5.94±0.26) ng/L,respectively,from individuals with mild, moderate and severe hepatitis (P<0.01). Conclusion The Fas-FasL mediated apoptosis might play an important role in the disease progress of ASC.
