食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
1期
76-79
,共4页
罗晓琴%赵正苗%吴继华%刘叶%刘琳
囉曉琴%趙正苗%吳繼華%劉葉%劉琳
라효금%조정묘%오계화%류협%류림
黄曲霉毒素M1%快速检测%胶体金免疫层析%试纸条%牛奶
黃麯黴毒素M1%快速檢測%膠體金免疫層析%試紙條%牛奶
황곡매독소M1%쾌속검측%효체금면역층석%시지조%우내
aflatoxinM1%rapid detection%gold immunochromatography assay%strip%milk
应用胶体金免疫层析技术,建立一种快速检测牛奶中黄曲霉毒素M1的方法。采用柠檬酸三钠还原法制备胶体金颗粒,单克隆抗体胶体金标记物的制备并冻干成微孔试剂,样品吸收垫和反应膜的制备,试纸条的组装。所研制的黄曲霉毒素M1快速检测试纸条的检测限为0.3μg/L,检测时间为10 min,假阳性率和假阴性率为0。试纸条使用简单方便,适合进行现场快速检测牛奶中残留的黄曲霉毒素M1。
應用膠體金免疫層析技術,建立一種快速檢測牛奶中黃麯黴毒素M1的方法。採用檸檬痠三鈉還原法製備膠體金顆粒,單剋隆抗體膠體金標記物的製備併凍榦成微孔試劑,樣品吸收墊和反應膜的製備,試紙條的組裝。所研製的黃麯黴毒素M1快速檢測試紙條的檢測限為0.3μg/L,檢測時間為10 min,假暘性率和假陰性率為0。試紙條使用簡單方便,適閤進行現場快速檢測牛奶中殘留的黃麯黴毒素M1。
응용효체금면역층석기술,건립일충쾌속검측우내중황곡매독소M1적방법。채용저몽산삼납환원법제비효체금과립,단극륭항체효체금표기물적제비병동간성미공시제,양품흡수점화반응막적제비,시지조적조장。소연제적황곡매독소M1쾌속검측시지조적검측한위0.3μg/L,검측시간위10 min,가양성솔화가음성솔위0。시지조사용간단방편,괄합진행현장쾌속검측우내중잔류적황곡매독소M1。
To establish a rapid assay for detection of aflatoxin M1, gold immunochromatographyassay (GICA) was studied. Colloida lgold marked coupled with monoelonal antibody against aflatoxin M1 and freeze into microporous reagents, sample absorption cushion and the reaction film preparation,installation of the strip.The results showed that the detection limit was 0.3 μg/L in milk. And the detection timewithin 10 min. There isnofalse positiveandfalsenegativeresults. The strip is very simpleandconvenientto rapidlydetect and screen of aflatoxin M1 in milk.