CAJ | 학술논문

应用胶体金免疫层析技术，建立一种快速检测牛奶中黄曲霉毒素M1的方法。采用柠檬酸三钠还原法制备胶体金颗粒，单克隆抗体胶体金标记物的制备并冻干成微孔试剂，样品吸收垫和反应膜的制备，试纸条的组装。所研制的黄曲霉毒素M1快速检测试纸条的检测限为0.3μg/L，检测时间为10 min，假阳性率和假阴性率为0。试纸条使用简单方便，适合进行现场快速检测牛奶中残留的黄曲霉毒素M1。
응용효체금면역층석기술，건립일충쾌속검측우내중황곡매독소M1적방법。채용저몽산삼납환원법제비효체금과립，단극륭항체효체금표기물적제비병동간성미공시제，양품흡수점화반응막적제비，시지조적조장。소연제적황곡매독소M1쾌속검측시지조적검측한위0.3μg/L，검측시간위10 min，가양성솔화가음성솔위0。시지조사용간단방편，괄합진행현장쾌속검측우내중잔류적황곡매독소M1。
To establish a rapid assay for detection of aflatoxin M1, gold immunochromatographyassay (GICA) was studied. Colloida lgold marked coupled with monoelonal antibody against aflatoxin M1 and freeze into microporous reagents, sample absorption cushion and the reaction film preparation,installation of the strip.The results showed that the detection limit was 0.3 μg/L in milk. And the detection timewithin 10 min. There isnofalse positiveandfalsenegativeresults. The strip is very simpleandconvenientto rapidlydetect and screen of aflatoxin M1 in milk.