CAJ | 학술논문

以羟基自由基、超氧自由基清除能力、抗蛋白质糖基化反应及D-半乳糖氧化模型小鼠血液和肝脏中的MDA含量和GSH-Px活力的影响为指标，考察鳙鱼活性肽体内外清除自由基的能力。鳙鱼活性肽具有较强的清除·OH能力，清除率与浓度呈剂量关系，并能明显降低邻苯三酚自氧化速率，抑制率与浓度呈正相关，但与肌肽比较，鳙鱼活性肽的体外抗氧化能力相对要弱。体内试验表明，鳙鱼活性肽可以显著降低D-半乳糖氧化模型小鼠血液和肝脏中的MDA含量，显著提高GSH-Px活力，说明鳙鱼活性肽具有较好的体内抗氧化作用。
이간기자유기、초양자유기청제능력、항단백질당기화반응급D-반유당양화모형소서혈액화간장중적MDA함량화GSH-Px활력적영향위지표，고찰용어활성태체내외청제자유기적능력。용어활성태구유교강적청제·OH능력，청제솔여농도정제량관계，병능명현강저린분삼분자양화속솔，억제솔여농도정정상관，단여기태비교，용어활성태적체외항양화능력상대요약。체내시험표명，용어활성태가이현저강저D-반유당양화모형소서혈액화간장중적MDA함량，현저제고GSH-Px활력，설명용어활성태구유교호적체내항양화작용。
In this paper, using hydroxyl radical, superoxide radical scavenging, anti-protein glycosylation reactions, the blood and liver MDA content of D-galactose oxidase mice and GSH-Px activity as the indicators, the vivo and vitro antioxidant activity of active polypeptide from Hypophthalmichthys nobilis has been investigated. The results show that bighead active polypeptide has strong scavenging ability, its clearance rate has relationship with dose; and can significantly reduce the pyrogallol autoxidation rate, the inhibition rate is positively correlated with the concentration, but compared with carnosine, bighead active peptide in vitro antioxidant capacity is relatively weak. Internal tests show that bighead active peptides can significantly reduce the blood and liver MDA content of D-galactose oxidase mice, significantly increased GSH-Px activity, which indicated bighead active polypeptide has good internal antioxidant activity.