CAJ | 학술논문

以人工养殖达氏鳇脊骨为原料，按碱提-酶解-乙醇沉淀工艺流程提取硫酸软骨素的工艺条件进行研究。通过单因素及正交试验，得出碱提的最佳条件是碱液质量分数为6%，料液比为1∶7，提取温度为40℃，提取时间为4.5 h。酶解的最佳条件为浸提液pH值调至8~9，采用质量比1∶1的木瓜蛋白酶和胰蛋白酶的混合酶，按质量分数2.5%加入，55℃水浴酶解5 h。通过单因素试验，得出乙醇沉淀的最佳条件为滤液pH值调为6.5，加入无水乙醇至醇沉体积分数70%，室温静置过夜。在最佳工艺条件下硫酸软骨素得率为40.65%， D-葡萄糖醛酸含量为22.15%。
이인공양식체씨황척골위원료，안감제-매해-을순침정공예류정제취류산연골소적공예조건진행연구。통과단인소급정교시험，득출감제적최가조건시감액질량분수위6%，료액비위1∶7，제취온도위40℃，제취시간위4.5 h。매해적최가조건위침제액pH치조지8~9，채용질량비1∶1적목과단백매화이단백매적혼합매，안질량분수2.5%가입，55℃수욕매해5 h。통과단인소시험，득출을순침정적최가조건위려액pH치조위6.5，가입무수을순지순침체적분수70%，실온정치과야。재최가공예조건하류산연골소득솔위40.65%， D-포도당철산함량위22.15%。
The chondroitin sulfate from kaluga sturgeon is extracted by alkali-enzyme hydrolysis and ethanol precipitation. After single factor and orthogonal test, the optimal extraction is obtained as follows: the alkali concentration is 6%, the solid-liquid ratio is 1∶7, the extraction temperature is 40 ℃, the extraction time is 4.5 h. While the optimal conditions for enzymatic hydrolysis is that the pH of extract solution is adjusted from 8~9, the ratio of papain and trypsin is 1∶1, the addtion of enzyme mixtures is 2.5%, the temperature of enzyme is 55℃, the hydrolysis time is 5 h. The optimal ethanol precipitation is obtained by single factor test, the pH of filtrate solution is 6.5, the ethanol concentration is 70% at room temperature overnight. The yield of chondroitin sulfate under the optimum conditions is 40.65%, D-glucuronic acid content of 22.15%.