CAJ | 학술논문

目的观察微小RNA(miRNA)-375在食管鳞状细胞癌(ESCC)中的表达,探讨其启动子区的甲基化水平与ESCC发生的关系.方法对62例ESCC组织标本和正常食管组织(距癌肿边缘＞3 cm)进行实时荧光定量聚合酶链反应(FQ-PCR)检测,使用甲基化特异性聚合酶链反应(MSP)检测样本中miRNA-375上游启动子区的甲基化.统计学分析miRNA-375的表达与临床因素的相关性.结果 62例ESCC组织标本中有53例miRNA-375E呈明显低表达(85％).MSP结果显示,ESCC组织的甲基化水平明显高于正常食管组织,差异有统计学意义(P＜0.05).与临床因素的相关分析显示,miRNA-375在ESCC组织中低表达,与ESCC的TNM分期呈负相关(P＜0.05).结论 ESCC组织中存在miRNA-375基因异常低表达及其启动子区的高甲基化,可能在ESCC发生发展中发挥重要作用.
목적 관찰미소RNA(miRNA)-375재식관린상세포암(ESCC)중적표체,탐토기계동자구적갑기화수평여ESCC발생적관계.방법 대62례ESCC조직표본화정상식관조직(거암종변연＞3 cm)진행실시형광정량취합매련반응(FQ-PCR)검측,사용갑기화특이성취합매련반응(MSP)검측양본중miRNA-375상유계동자구적갑기화.통계학분석miRNA-375적표체여림상인소적상관성.결과 62례ESCC조직표본중유53례miRNA-375E정명현저표체(85％).MSP결과현시,ESCC조직적갑기화수평명현고우정상식관조직,차이유통계학의의(P＜0.05).여림상인소적상관분석현시,miRNA-375재ESCC조직중저표체,여ESCC적TNM분기정부상관(P＜0.05).결론 ESCC조직중존재miRNA-375기인이상저표체급기계동자구적고갑기화,가능재ESCC발생발전중발휘중요작용.
Objective To investigate the expression of microRNA (miRNA)-375 and its genomic promoter DNA methylation in esophageal squamous cell carcinoma (ESCC).Methods The expression of miRNA-375 in ESCC and the matched normal esophageal tissues (from the tumor edge ＞ 3 cm) was detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR).DNA methylation status was examined by methylation specific polymerease chain reaction (MSP).The association between miRNA-375 expression and clinical factors were analyzed statistically.Results As compared with normal controls,miRNA-375 was significantly decreased in ESCC.The methylation levels in ESCC tissues were significantly higher than in the matched normal esophageal tissues (P ＜ 0.05).The low expression of miRNA-375 was significandy associated with ESCC TNM stage (P ＜ 0.05).Conclusion The lower expression of miRNA-375 and aberrant hypermethylation of the CpG island promoters in ESCC may play a crucial role in the carcinogenesis of ESCC.