CAJ | 학술논문

以继代的粳粳交（空育131/稻花香2号）F1花药培养的愈伤组织为试验材料，研究培养基中的影响因素，对已有的培养基进行优化。共用20种培养基进行分化，涉及7种影响因素：谷氨酰胺，糖类，激素，山梨醇，铜、银离子组合，硅素和活性炭。结果表明：（1）长时间继代的愈伤组织仍具有分化能力。（2）可以显著提高绿苗分化率的因素为60 mg/L硅素，0．25 mg/L AgNO3和0．75 mg/L CuSO4·5H2O。（3）加160目活性炭的培养基中愈伤生长状况得到很大改善。（4）最佳分化培养基为G1W3。
이계대적갱갱교（공육131/도화향2호）F1화약배양적유상조직위시험재료，연구배양기중적영향인소，대이유적배양기진행우화。공용20충배양기진행분화，섭급7충영향인소：곡안선알，당류，격소，산리순，동、은리자조합，규소화활성탄。결과표명：（1）장시간계대적유상조직잉구유분화능력。（2）가이현저제고록묘분화솔적인소위60 mg/L규소，0．25 mg/L AgNO3화0．75 mg/L CuSO4·5H2O。（3）가160목활성탄적배양기중유상생장상황득도흔대개선。（4）최가분화배양기위G1W3。
The influencing factors of the medium were optimized for the differentiation of subcultured anther calli, which were derived from japonica/japonica hybrid F1 (Kongyu 131/Daohuaxiang 2). 20 culture mediums were devised for callus differentiation. Different combinations of 7 important factors were considered for the medium design. The 7 important factors included glutamine, carbohydrates, hormones, sorbitol, the composition of copper and silver ion, silicone, and activated charcoal. The results showed that: (1) The prolonged subcultured calli continued to possess the differentiation ability. (2) The factor combination with 60 mg/L Silicon, 0.25 mg/L AgNO3 and 0.75mg/L CuSO45H2O were found to have the potential in significantly improving the differenti`ation rate. (3) The callus growth conditions have been greatly improved through adding 160-mesh activated charcoal. (4) The best medium to differentiate subcultured calli was G1W3.