CAJ | 학술논문

目的：探讨球药隔重楼中分离得到的球药隔重楼凝集素（PFL）的肿瘤细胞毒性及其可能的机制。方法用交换层析法纯化PFL同源二聚体；通过噻唑蓝（MTT）法、形态学观察、乳酸脱氢酶（LDH）法、糖蛋白阻断分析等方法分析PFL对肿瘤细胞增殖的抑制情况及其机制。结果 PFL对宫颈癌 HeLa、肝癌 HepG2以及鼻咽癌CNE-2细胞增殖都有抑制作用。PFL的糖结合位点被甲状腺球蛋白阻断后对肿瘤细胞生长的抑制活性明显降低，表明PFL是通过识别 CNE-2细胞表面的糖链作用于细胞，并诱导细胞凋亡。半胱氨酸蛋白酶（caspase）家族抑制剂的研究表明 PFL是通过经典的 caspase途径诱导 CNE-2细胞凋亡。结论 PFL可通过 caspase途径诱导肿瘤细胞凋亡。
목적：탐토구약격중루중분리득도적구약격중루응집소（PFL）적종류세포독성급기가능적궤제。방법용교환층석법순화PFL동원이취체；통과새서람（MTT）법、형태학관찰、유산탈경매（LDH）법、당단백조단분석등방법분석PFL대종류세포증식적억제정황급기궤제。결과 PFL대궁경암 HeLa、간암 HepG2이급비인암CNE-2세포증식도유억제작용。PFL적당결합위점피갑상선구단백조단후대종류세포생장적억제활성명현강저，표명PFL시통과식별 CNE-2세포표면적당련작용우세포，병유도세포조망。반광안산단백매（caspase）가족억제제적연구표명 PFL시통과경전적 caspase도경유도 CNE-2세포조망。결론 PFL가통과 caspase도경유도종류세포조망。
Objective To explore the antineoplastic activity of paris fargesii franch lectin(PFL)and its mechanism.Methods PFL was purified by exchange chromatography;MTT method,cell morphological analysis,LDH activity-based cytotoxicity assays and caspase inhibitors analyses were adopted to analyze the obj ects mentioned above.Results PFL exhibited remarkable inhibitory effect on HeLa,HepG2 and CNE-2 cells and significantly decreased CNE-2 cell apoptosis in dose-dependent manner.The cytotoxici-ty was abruptly decreased when the carbohydrate binding-specific activity was inhibited,suggesed a significant correlation between carbohydrate binding-specific activity and the antineoplastic mechanism.The research of caspase in bibitory shnoed that PEL in-duced apoptosis of CNE-2 cells through the dassic caspase pathway.Conclusion PFL induces the cancer cell apoptosis,and caspase may play an important role in this apoptotic mechanism.