华南师范大学学报（自然科学版）
華南師範大學學報（自然科學版）
화남사범대학학보（자연과학판）
JOURNAL OF SOUTH CHINA NORMAL UNIVERSITY （NATURAL SCIENCE EDITION）
2014年
1期
77-82
,共6页
扇形游仆虫%谷胱甘肽过氧化物酶%cDNA克隆%序列特征
扇形遊僕蟲%穀胱甘肽過氧化物酶%cDNA剋隆%序列特徵
선형유부충%곡광감태과양화물매%cDNA극륭%서렬특정
Euplotes vannus%glutathione peroxidase%cDNA cloning%sequence characterization
采用cDNA末端快速扩增（rapid amplification of cDNA ends，RACE）技术，对纤毛虫原生动物扇形游仆虫（Eu-plotes vannus）谷胱甘肽过氧化物酶（ GPx） cDNA特征进行分析。获得扇形游仆虫GPx cDNA全长序列，为672 bp，编码182个氨基酸，属硫氧还蛋白超家族，含有3个催化残基和3个特征性基序。氨基酸序列与其他纤毛虫物种同源性较高，亲缘关系较近。
採用cDNA末耑快速擴增（rapid amplification of cDNA ends，RACE）技術，對纖毛蟲原生動物扇形遊僕蟲（Eu-plotes vannus）穀胱甘肽過氧化物酶（ GPx） cDNA特徵進行分析。穫得扇形遊僕蟲GPx cDNA全長序列，為672 bp，編碼182箇氨基痠，屬硫氧還蛋白超傢族，含有3箇催化殘基和3箇特徵性基序。氨基痠序列與其他纖毛蟲物種同源性較高，親緣關繫較近。
채용cDNA말단쾌속확증（rapid amplification of cDNA ends，RACE）기술，대섬모충원생동물선형유부충（Eu-plotes vannus）곡광감태과양화물매（ GPx） cDNA특정진행분석。획득선형유부충GPx cDNA전장서렬，위672 bp，편마182개안기산，속류양환단백초가족，함유3개최화잔기화3개특정성기서。안기산서렬여기타섬모충물충동원성교고，친연관계교근。
Glutathione peroxidase ( GPx) has been widely used as a biomarker of environmental stress in ecotoxi-cology.In this study , GPx gene in Euplotes vannus was cloned and characterized .Degenerate primers were de-signed according to the glutathione peroxidase amino acid sequences in conservative area from similar species .The full-length cDNA was cloned from E.vannus using RACE-PCR.Results showed that the complete cDNA in E.van-nus is 672 bp encoding 182 amino acids and belongs to thioredoxin-like superfamily .Analyses of the derived amino acid sequence showed that GPx protein contains conservative structures , such as conserved catalytic residues and characteristic motifs , which is typical of glutathione peroxidase .Phylogenic analysis of amino acid sequences showed that E.vannus shares high homology and near affinity with other ciliate species .
