CAJ | 학술논문

为了探究重复超排对小尾寒羊超排效果的影响，以30只2～5岁的小尾寒羊为研究对象，首次超排注射8 mg卵泡刺激素（FSH ），根据超排反应将供体羊分为3组（一组反应差，二组反应正常，三组反应剧烈），分别对每组第2次、第3次重复超排的激素用量进行调整。一组第2次重复超排时，增加2 mg卵泡刺激素（FSH）及200 IU孕马血清素（PMSG），第3次激素用量同第2次；二组第2次增加2 mg FSH ，第3次增加4 mg FSH ；三组第2次减少1 mg FSH ，第3次减少1.5 mg FS H。结果显示，一组在第2次超排时平均红体数由2.00个增加到7.40个，增加270.0％，平均回收卵子数由1.60枚增加到5.50枚，增加243.8％，第3次超排平均红体数为7.80个，比首次增加290.0％，平均回收卵子数为4.60枚，比首次增加187.5％，第2、3次超排的平均红体数和平均回收卵子数均显著高于首次；二组在调整激素用量后保持稳定的超排效果，第2、3次超排结果与首次差异不显著；三组在调整激素用量后超排效果依然不佳，3次超排结果差异不显著。表明，根据首次超排效果调整小尾寒羊重复超排的激素用量方法可行，对于首次超排反应差的个体，重复超排时增加2 mg FS H、添加200 IU PM SG ，可显著提高平均红体数和回收卵子数；对于首次超排效果好的个体重复超排时增加2 mg FS H用量，能保证供体重复超排的稳定性。
위료탐구중복초배대소미한양초배효과적영향，이30지2～5세적소미한양위연구대상，수차초배주사8 mg란포자격소（FSH ），근거초배반응장공체양분위3조（일조반응차，이조반응정상，삼조반응극렬），분별대매조제2차、제3차중복초배적격소용량진행조정。일조제2차중복초배시，증가2 mg란포자격소（FSH）급200 IU잉마혈청소（PMSG），제3차격소용량동제2차；이조제2차증가2 mg FSH ，제3차증가4 mg FSH ；삼조제2차감소1 mg FSH ，제3차감소1.5 mg FS H。결과현시，일조재제2차초배시평균홍체수유2.00개증가도7.40개，증가270.0％，평균회수란자수유1.60매증가도5.50매，증가243.8％，제3차초배평균홍체수위7.80개，비수차증가290.0％，평균회수란자수위4.60매，비수차증가187.5％，제2、3차초배적평균홍체수화평균회수란자수균현저고우수차；이조재조정격소용량후보지은정적초배효과，제2、3차초배결과여수차차이불현저；삼조재조정격소용량후초배효과의연불가，3차초배결과차이불현저。표명，근거수차초배효과조정소미한양중복초배적격소용량방법가행，대우수차초배반응차적개체，중복초배시증가2 mg FS H、첨가200 IU PM SG ，가현저제고평균홍체수화회수란자수；대우수차초배효과호적개체중복초배시증가2 mg FS H용량，능보증공체중복초배적은정성。
30 Small Tailed Han Sheep at the age of 2-5 years had been taken as the research ob-ject to investigate the effect of repeated super-ovulation .8 mg FSH was injected in the first su-per-ovulation .The donor sheep were dived into three groups according to the first super-ovulation reaction and the number of ova collected .Reaction of group 1 ,group 2 ,group 3 were weak ,nor-mal ,and serious respectively .The hormone dosage would be adjusted in the followed super-ovula-tion .2 mg FSH and 200 IU PMSG were increased for the second time in the first group .The hor-mone dosage for the third time was the same to the second time .2 mg FSH was increased for the second time and 4 mg FSH was increased for the third time in the second group .1 mg and 1 .5 mg FSH was decreased for the second and third time respectively in the third group .The results indi-cated that the average amount of corpora luteum increased from 2 .00 to 7 .40 ,increasing by 270.0% .The amount of ova collected increased from 1 .60 to 5 .50 ,increasing by 243 .80 .0% .The average amount of corpora luteum was 7 .80 in the third super-ovulation ,increasing by 290 .0%compared with that of the first time .The amount of ova collected was 4 .60 ,increasing by 187.5%compared with that of the first time .The amount of corpora luteum and ova collected in the se-cond and the third time were significantly more than that of the first time .Effect of the adjusted hormone dosage was stable in the second group .The results of the super-ovulation for the second and third time had no significant difference between that of the first .The method to adjust the hormone dosage in Small Tail Han Sheep according to the first super-ovulation reaction was avai-lable .The amount of corpora luteum and ova collected in the groups that reacted weak added sig-nificantly increasing 2 mg FSH and 200 IU PMSG in repeated super-ovulation .The stabilization of repeated super-ovulation could be assured increasing 2 mg FSH in the repeated super-ovulation that reacted normal .