CAJ | 학술논문

为了研究中国莲小分子热激蛋白的生理功能，通过RACE技术克隆得到小分子热激蛋白基因HSP17.5全长cDNA。该基因开放阅读框全长471bp，编码156个氨基酸，推测其分子量为17.5kD，等电点5.94。半定量RTPCR试验结果显示，HSP17.5在中国莲授粉后不同时期的胚轴中均有表达，表达量呈现先降低后恢复的特点。HSP17.5在授粉后10d的子叶中有低丰度表达，之后消失。表明HSP17．5蛋白在中国莲种子萌发和幼苗早期的形态建成中可能起着重要的调节作用。
위료연구중국련소분자열격단백적생리공능，통과RACE기술극륭득도소분자열격단백기인HSP17.5전장cDNA。해기인개방열독광전장471bp，편마156개안기산，추측기분자량위17.5kD，등전점5.94。반정량RTPCR시험결과현시，HSP17.5재중국련수분후불동시기적배축중균유표체，표체량정현선강저후회복적특점。HSP17.5재수분후10d적자협중유저봉도표체，지후소실。표명HSP17．5단백재중국련충자맹발화유묘조기적형태건성중가능기착중요적조절작용。
A full length cDNA of small heat shock protein gene HSP17 .5 was isolated from sa-cred lotus by RACE method .The ORF of HSP17 .5 was 471 bp ,encodes 156 amino acid resi-dues .The deduced molecular weight and PI was 17 .5 kD and 5 .94 ,respectively .The semi-quanti-tative RT PCR result indicated that HSP17 .5 was expressed in embryonal axis during all the development period after pollination ,and the expression level was in“descent-recuperation”pat-tern .The HSP17 .5 was lowly expressed in cotyledon at 10 days after pollination ,and then disap-peared .HSP17 .5 may play an important role in seed germination and early seedling morphogenesis .